College of Veterinary Medicine, Chonnam National University, Gwangju, Republic of Korea.
J Med Food. 2009 Dec;12(6):1353-8. doi: 10.1089/jmf.2008.1322.
The protective properties of an herbal preparation (HemoHIM) against intestinal damage were examined by evaluating its effects on jejunal crypt survival, morphological changes, and apoptosis in gamma-irradiated mice. The mice were whole-body irradiated with 12 Gy for the examination of jejunal crypt survival and any morphological changes and with 2 Gy for the detection of apoptosis and Ki-67 labeling. Irradiation was conducted using (60)Co gamma-rays. HemoHIM treatment was administered intraperitonially at a dosage of 50 mg/kg of body weight at 36 and 12 hours pre-irradiation and 30 minutes post-irradiation or orally at a dosage of 250 mg/kg of body weight/day for 7 or 11 days before necropsy. The HemoHIM-treated group displayed a significant increase in survival of jejunal crypts, when compared to the irradiation controls. HemoHIM treatment decreased intestinal morphological changes such as crypt depth, villus height, mucosal length, and basal lamina length of 10 enterocytes after irradiation. Furthermore, the administration of HemoHIM protected intestinal cells from irradiation-induced apoptosis. These results suggested that HemoHIM may be therapeutically useful to reduce intestinal injury following irradiation.
研究了一种草药制剂（HemoHIM）对肠道损伤的保护作用，评估了其对γ射线照射小鼠空肠隐窝存活、形态变化和细胞凋亡的影响。采用（60）Coγ射线对小鼠进行全身照射，剂量为 12 Gy，用于检测空肠隐窝存活和任何形态变化，剂量为 2 Gy 用于检测细胞凋亡和 Ki-67 标记。HemoHIM 治疗在照射前 36 小时和 12 小时以及照射后 30 分钟时以 50 mg/kg 体重的剂量腹腔内给药，或在剖检前 7 天或 11 天以 250 mg/kg 体重/天的剂量口服给药。与照射对照组相比，HemoHIM 处理组空肠隐窝的存活率显著增加。HemoHIM 治疗可减少辐射后隐窝深度、绒毛高度、粘膜长度和 10 个肠上皮细胞的基底层长度等肠道形态变化。此外，HemoHIM 的给药可保护肠道细胞免受辐射诱导的凋亡。这些结果表明，HemoHIM 可能对减少辐射引起的肠道损伤具有治疗作用。
