Laboratoire d'Ecologie Alpine (LECA, UMR 5553 CNRS-Université) Grenoble, France.
Insect Mol Biol. 2010 Apr;19(2):185-93. doi: 10.1111/j.1365-2583.2009.00967.x. Epub 2009 Dec 21.
Transcription profiles of 11 Aedes aegypti P450 genes from CYP6 and CYP9 subfamilies potentially involved in xenobiotic metabolism were investigated. Many genes were preferentially transcribed in tissues classically involved in xenobiotic metabolism including midgut and Malpighian tubules. Life-stage transcription profiling revealed important variations amongst larvae, pupae, and adult males and females. Exposure of mosquito larvae to sub-lethal doses of three xenobiotics induced the transcription of several genes with an induction peak after 48 to 72 h exposure. Several CYP genes were also induced by oxidative stress and one gene strongly responded to 20-hydroxyecdysone. Overall, this study revealed that these P450s show different transcription profiles according to xenobiotic exposures, life stages or sex. Their putative chemoprotective functions are discussed.
本研究调查了 11 种埃及伊蚊 P450 基因（CYP6 和 CYP9 亚家族）的转录谱，这些基因可能参与了外来化合物的代谢。许多基因在经典的参与外来化合物代谢的组织中优先转录，包括中肠和马氏管。生活史转录谱分析显示，幼虫、蛹和成年雌雄蚊之间存在重要差异。蚊子幼虫暴露于三种亚致死剂量的外源化学物质后，几种基因的转录水平在暴露后 48 至 72 小时达到峰值。氧化应激也诱导了几个 CYP 基因的表达，其中一个基因对 20-羟基蜕皮酮的反应非常强烈。总的来说，这项研究表明，这些 P450 根据外源化学物质暴露、生活史或性别表现出不同的转录谱。讨论了它们可能的化学保护功能。
