Department of Biological Sciences & Biomolecular Sciences Institute, Florida International University, Miami, Florida, United States of America.
PLoS One. 2023 Oct 24;18(10):e0293018. doi: 10.1371/journal.pone.0293018. eCollection 2023.
Modulation of odorant receptors mRNA induced by prolonged odor exposure is highly correlated with ligand-receptor interactions in Drosophila as well as mammals of the Muridae family. If this response feature is conserved in other organisms, this presents an intriguing initial screening tool when searching for novel receptor-ligand interactions in species with predominantly orphan olfactory receptors. We demonstrate that mRNA modulation in response to 1-octen-3-ol odor exposure occurs in a time- and concentration-dependent manner in Aedes aegypti mosquitoes. To investigate gene expression patterns at a global level, we generated an odor-evoked transcriptome associated with 1-octen-3-ol odor exposure. Transcriptomic data revealed that ORs and OBPs were transcriptionally responsive whereas other chemosensory gene families showed little to no differential expression. Alongside chemosensory gene expression changes, transcriptomic analysis found that prolonged exposure to 1-octen-3-ol modulated xenobiotic response genes, primarily members of the cytochrome P450, insect cuticle proteins, and glucuronosyltransferases families. Together, these findings suggest that mRNA transcriptional modulation of olfactory receptors caused by prolonged odor exposure is pervasive across taxa and can be accompanied by the activation of xenobiotic responses.
长时程气味暴露诱导气味受体 mRNA 的调制与果蝇以及鼠科哺乳动物的配体-受体相互作用高度相关。如果这种反应特征在其他生物中保守存在，那么当在主要具有孤儿嗅觉受体的物种中寻找新的受体-配体相互作用时，这将提供一个有趣的初始筛选工具。我们证明，在埃及伊蚊中，1-辛烯-3-醇气味暴露引起的 mRNA 调制以时间和浓度依赖的方式发生。为了在全局水平上研究基因表达模式，我们生成了与 1-辛烯-3-醇气味暴露相关的气味诱导转录组。转录组数据显示，ORs 和 OBPs 转录响应，而其他化学感觉基因家族显示出很少或没有差异表达。除了化学感觉基因表达变化外，转录组分析还发现，长时间暴露于 1-辛烯-3-醇会调节外源物反应基因，主要是细胞色素 P450、昆虫表皮蛋白和葡萄糖醛酸转移酶家族的成员。总之，这些发现表明，长时程气味暴露引起的嗅觉受体 mRNA 转录调节是跨类群普遍存在的，并且可以伴随着外源物反应的激活。
