Rickwood D, MacGillivray A J, Whish W J
Eur J Biochem. 1977 Oct 3;79(2):589-98. doi: 10.1111/j.1432-1033.1977.tb11843.x.
When incubated in vitro purified mouse nuclei incorporate NAD into poly(ADP-Rib). Analysis of the product on CsDl/urea gradients showed that a large proportion of the poly(ADP-Rib) was not attached to protein. The free poly(ADP-Rib) did not appear to arise through degradation and its chain length was significantly longer than the poly(ADP-Rib) attached to proteins. Fractionation of the proteins on hydroxyapatite revealed that tissue-specific modification of both the histones and non-histone proteins, had occurred. In the case of one protein species there was evidence for the existence of several forms with different numbers of ADP-Rib residues.
体外培养时，纯化的小鼠细胞核将NAD掺入聚（ADP-核糖）中。在CsDl/尿素梯度上对产物进行分析表明，大部分聚（ADP-核糖）未与蛋白质结合。游离的聚（ADP-核糖）似乎不是由降解产生的，其链长明显长于与蛋白质结合的聚（ADP-核糖）。用羟基磷灰石对蛋白质进行分级分离显示，组蛋白和非组蛋白都发生了组织特异性修饰。对于一种蛋白质，有证据表明存在几种具有不同数量ADP-核糖残基的形式。
