黏菌盘基网柄菌细胞核中聚（ADP - 核糖）聚合酶活性的表征
Characterisation of poly(ADP-Rib) polymerase activity in nuclei from the slime mould Dictyostelium discoideum.
作者信息
Rickwood D, Osman M S
出版信息
Mol Cell Biochem. 1979 Oct 15;27(2):79-84. doi: 10.1007/BF00218351.
PMID:228176
Abstract
Nuclei isolated from D. discoideum and incubated in vitro with 3H-NAD synthesise poly(ADP-Rib). The optimum incubation conditions for the poly(ADP-Rib) polymerase were determined. The Km of the enzyme is 18 microM NAD and it is inhibited by nicotinamide. Most of the poly(ADP-Rib) synthesised is attached to nuclear proteins.
摘要
从盘基网柄菌中分离出的细胞核，在体外与3H-NAD一起温育时会合成聚（ADP-核糖）。确定了聚（ADP-核糖）聚合酶的最佳温育条件。该酶的Km为18微摩尔NAD，并且它受到烟酰胺的抑制。合成的大部分聚（ADP-核糖）都附着在核蛋白上。
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