Department of Oral and Maxillofacial Surgery, Ninth People's Hospital, School of Stomatology, Shanghai Jiao Tong University School of Medicine, No. 639 Zhizaoju Road, Shanghai 200011, China.
Int J Oral Maxillofac Surg. 2010 Feb;39(2):174-81. doi: 10.1016/j.ijom.2009.11.018. Epub 2009 Dec 29.
Previously, an in vitro cellular carcinogenesis model of oral squamous cell carcinoma (OSCC) was established with a line of human immortalized oral epithelial cells (HIOECs), a line of cancerous HB96 cells and another type of cell (HB56 cells) at the early stage of carcinogenesis. In this study, comparative proteomic analysis identified a panel of differentially expressed proteins among these cells. Cathepsin B was one of the significantly up-regulated proteins accompanying cellular transformation. Cathepsin B was further validated for its expression in the three cell lines and in clinical samples of tumour tissues and their adjacent normal epithelia from 30 primary OSCC patients. Western blot analysis and real-time PCR detected increased Cathepsin B protein and mRNA levels in the cancerous HB56 and HB96 cells over HIOECs. Immunohistochemistry and real-time PCR showed elevated Cathepsin B protein and mRNA levels in the tumour tissues over the adjacent non-malignant epithelia from OSCC patients. The results presented here suggest that the expression of Cathepsin B increases along with the cancerisation in OSCC both in vitro and in vivo, and it may serve as a candidate biomarker of OSCC.
先前，我们建立了一个体外口腔鳞状细胞癌（OSCC）的细胞癌变模型，该模型使用了一条人永生化口腔上皮细胞（HIOECs）、一条癌变的 HB96 细胞系和另一种处于癌变早期的细胞系（HB56 细胞）。在这项研究中，比较蛋白质组学分析在这些细胞中鉴定出了一组差异表达的蛋白质。组织蛋白酶 B 是伴随细胞转化而上调的显著蛋白之一。组织蛋白酶 B 的表达在这三种细胞系以及 30 位原发性 OSCC 患者的肿瘤组织及其相邻正常上皮的临床样本中得到了进一步验证。Western blot 分析和实时 PCR 检测到癌变的 HB56 和 HB96 细胞中的 Cathepsin B 蛋白和 mRNA 水平高于 HIOECs。免疫组织化学和实时 PCR 显示，OSCC 患者肿瘤组织中的 Cathepsin B 蛋白和 mRNA 水平高于相邻的非恶性上皮组织。本研究结果表明，组织蛋白酶 B 的表达在 OSCC 中无论是在体外还是体内，都随着癌变而增加，它可能成为 OSCC 的候选生物标志物。
