以聚甘油为核的 PEI 接枝超支化聚合物用于基因递送。
PEI grafted hyperbranched polymers with polyglycerol as a core for gene delivery.
机构信息
Key Laboratory of Biomedical Polymers of Ministry of Education, Department of Chemistry, Wuhan University, Wuhan, Hubei 430072, PR China.
出版信息
Colloids Surf B Biointerfaces. 2010 Apr 1;76(2):427-33. doi: 10.1016/j.colsurfb.2009.12.001. Epub 2009 Dec 11.
Hyperbranched polymers, PG6-PEI25k and PG6-PEI800, were synthesized through grafting branched polyethylenimines (PEIs) with molecular weights of 25 kDa and 800 Da to a polyglycerol core (PG6), respectively. The structure of the polymers was characterized by 1H NMR and FTIR. Through agarose gel electrophoresis retardation assay, PG6-PEI25k and PG6-PEI800 were demonstrated to have capability for DNA binding. PG6-PEI/DNA complexes with different weight ratios were characterized by TEM and particle size analysis. The activity of PG6-PEIs to mediate transfection of reporter plasmids pEGFP-C1 and pGL3-Luc was evaluated on 293T and HeLa cell lines. PG6-PEI25k and PG6-PEI800 showed enhanced levels in transgene expression and decreased cytotoxicities as compared with PEI25k and PEI800, respectively. The results indicated potential applications of PG6-PEIs for efficient gene delivery.
超支化聚合物 PG6-PEI25k 和 PG6-PEI800 分别通过将分子量为 25 kDa 和 800 Da 的支化聚乙烯亚胺（PEI）接枝到聚甘油核（PG6）上合成。聚合物的结构通过 1H NMR 和 FTIR 进行了表征。通过琼脂糖凝胶电泳阻滞实验，证明 PG6-PEI25k 和 PG6-PEI800 具有 DNA 结合能力。通过 TEM 和粒径分析对不同重量比的 PG6-PEI/DNA 复合物进行了表征。在 293T 和 HeLa 细胞系上评价了 PG6-PEIs 介导报告质粒 pEGFP-C1 和 pGL3-Luc 转染的活性。与 PEI25k 和 PEI800 相比，PG6-PEI25k 和 PG6-PEI800 表现出增强的转基因表达水平和降低的细胞毒性。结果表明 PG6-PEIs 具有高效基因传递的应用潜力。
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