Department of Clinical Sciences, Lund University Hospital, Sweden.
Cytokine. 2010 Mar;49(3):256-63. doi: 10.1016/j.cyto.2009.11.007. Epub 2009 Dec 29.
The lipopolysaccharide (LPS)-induced acute lung injury (ALI) model has been widely applied for pathophysiological and pharmacological research. The aim of present study is to understand the variation of acute pulmonary inflammation between mouse strains.
The present study investigated the susceptibility of acute production of inflammatory mediators, e.g. cytokines, chemokines and others, to LPS in C57BL/6J, Balb/cJ, DBA/1J, CD-1, NMRI, DBA/2J, A/J, and C3H/HeN mice.
The susceptibility to intra-tracheal challenge with LPS varied between measured variables, durations and strains. General lung hyper-reactive susceptibility to LPS-induced pulmonary production of 6-8 inflammatory mediators followed the order NMRI, Balb/cJ, C3H/HeN, A/J, C57BL/6J, DBA/1J, DBA/2J and CD-1 mice at 4h, and A/J, C3H/HeN, CD-1, NMRI, C57BL/6J, Balb/cJ, DBA/2J and DBA/1J mice at 24h.
Our data provide information for scientists to consider the proper strain of mice for the measurement of specific inflammatory mediators and to select sensitive or resistant mouse strains for understanding genetic variation in the pathogenesis and for the screening of target-oriented drug development.
脂多糖（LPS）诱导的急性肺损伤（ALI）模型已广泛应用于病理生理学和药理学研究。本研究旨在了解不同品系小鼠急性肺炎症的变化。
本研究调查了 C57BL/6J、Balb/cJ、DBA/1J、CD-1、NMRI、DBA/2J、A/J 和 C3H/HeN 小鼠对 LPS 诱导的急性炎症介质（如细胞因子、趋化因子等）产生的易感性。
对 LPS 气管内挑战的易感性因测量变量、持续时间和品系而异。一般来说，NMRI、Balb/cJ、C3H/HeN、A/J、C57BL/6J、DBA/1J、DBA/2J 和 CD-1 小鼠在 4 小时时对 LPS 诱导的肺内产生 6-8 种炎症介质的反应性较高，而在 24 小时时则为 A/J、C3H/HeN、CD-1、NMRI、C57BL/6J、Balb/cJ、DBA/2J 和 DBA/1J 小鼠。
我们的数据为科学家提供了信息，考虑使用适当的小鼠品系来测量特定的炎症介质，并选择敏感或抗性的小鼠品系，以了解发病机制中的遗传变异，并筛选靶向药物开发的目标。
