Department of Clinical Sciences, BMC F10, Lund University Hospital, Sweden.
Respir Physiol Neurobiol. 2010 Apr 30;171(2):157-64. doi: 10.1016/j.resp.2010.02.009. Epub 2010 Feb 25.
Clinical and experimental evidence suggests that genetic variations may play an important role in the development of acute lung injury (ALI). Lipopolysaccharide (LPS)-induced ALI models has been widely applied for pathophysiological and pharmacological research. In order to understand the variation of acute pulmonary reactions between mouse strains and find the optimal strain for target-oriented study, the present study investigated the alterations of acute lung hyperinflation, inflammation and injury in C57BL/6J, Balb/cJ, DBA/1J, CD-1, NMRI, DBA/2J, A/J and C3H/HeN mice after the intra-tracheal challenge with LPS. We found that LPS-induced ALI varied between measured variables, durations and strains. General score of LPS-induced acute lung hyperinflation, inflammation and edema followed the order CD-1, A/J, Balb/c, DBA/2J, C57BL/6J, DBA/1J, NMRI, C3H/HeN mice at 4h, and CD-1, C57BL/6J, Balb/c, C3H/HeN, NMRI, A/J, DBA/2J, DBA/1 mice at 24h. Thus, these data provide useful information to select sensitive or resistant strain mouse for understanding genetic variation of pathogenesis and screening of target-oriented drugs.
临床和实验证据表明，遗传变异可能在急性肺损伤（ALI）的发展中起重要作用。脂多糖（LPS）诱导的 ALI 模型已广泛应用于病理生理学和药理学研究。为了了解不同品系小鼠之间急性肺反应的差异，并找到适合目标导向研究的最佳品系，本研究探讨了 LPS 气管内给药后 C57BL/6J、Balb/cJ、DBA/1J、CD-1、NMRI、DBA/2J、A/J 和 C3H/HeN 小鼠急性肺充气过度、炎症和损伤的变化。结果发现，LPS 诱导的 ALI 在测量变量、持续时间和品系之间存在差异。LPS 诱导的急性肺充气过度、炎症和水肿的综合评分在 4 小时时依次为 CD-1、A/J、Balb/c、DBA/2J、C57BL/6J、DBA/1J、NMRI、C3H/HeN 小鼠，在 24 小时时依次为 CD-1、C57BL/6J、Balb/c、C3H/HeN、NMRI、A/J、DBA/2J、DBA/1 小鼠。因此，这些数据为选择敏感或抗性品系小鼠以了解发病机制的遗传变异和筛选靶向药物提供了有用的信息。
