Department of Anesthesiology, The First Affiliated Hospital, Guangxi Medical University, Nanning, Guangxi, PR China.
Int J Oncol. 2010 Feb;36(2):509-16.
The caudal-type homeobox gene Cdx2 encodes a transcription factor which in adult mammals is expressed in the cells of the intestinal epithelium and is thought to play an important role in their proliferation and differentiation. Cdx2 mediates apoptosis and suppresses tumorigenesis in many tissue types, but there are few data available on Cdx2 expression and its relationship to tumor kinetics in gastric cancer. To gain better insight into the involvement of Cdx2 in the biological characteristics of gastric cancer, we investigated the effect of Cdx2 overexpression on the progression of gastric carcinoma cells. A gastric cancer cell line stably overexpressing Cdx2 (MGC-803/Cdx2) was established. The influence of Cdx2 overexpression on in vitro cell growth was assessed by measuring cell survival, colony formation and cell cycle progression. The results clearly showed that overexpression of Cdx2 significantly inhibited cell growth and proliferation, blocking entry into the S phase of the cell cycle. MGC-803/Cdx2 cells also had a higher apoptotic rate than control cells. In addition, Cdx2 reduced the motility and invasion of gastric cancer cells. In summary, Cdx2 overexpression can effectively inhibit gastric cancer progression and may be used as a potent therapy.
尾型同源盒基因 Cdx2 编码一种转录因子，在成年哺乳动物中，该转录因子表达于肠上皮细胞中，并被认为在其增殖和分化中发挥重要作用。Cdx2 介导许多组织类型中的细胞凋亡并抑制肿瘤发生，但关于胃癌中 Cdx2 表达及其与肿瘤动力学的关系的数据很少。为了更深入地了解 Cdx2 在胃癌生物学特性中的作用，我们研究了 Cdx2 过表达对胃癌细胞进展的影响。建立了稳定过表达 Cdx2 的胃癌细胞系（MGC-803/Cdx2）。通过测量细胞存活、集落形成和细胞周期进程来评估 Cdx2 过表达对体外细胞生长的影响。结果清楚地表明，Cdx2 的过表达显著抑制了细胞生长和增殖，阻止细胞进入 S 期。MGC-803/Cdx2 细胞的凋亡率也明显高于对照细胞。此外，Cdx2 降低了胃癌细胞的迁移和侵袭能力。总之，Cdx2 的过表达可以有效抑制胃癌的进展，可能作为一种有效的治疗方法。
