Czygier Małgorzata, Ławicki Sławomir, Szmitkowski Maciej
Zakład Diagnostyki Biochemicznej Akademii Medycznej w Białymstoku, Białystok.
Przegl Lek. 2009;66(8):433-6.
G-CSF is a stimulatory factor of granulocyte colony formation. In vitro and in vivo G-CSF enhances functions of mature cells. Indirect evidence of this might be the increase of the activity of granulocyte enzymes participating in phagocytosis and killing tumor cells. The purpose of this investigation was to evaluate in the plasma of breast cancer patients the level of adhesion molecule - sL-selection, as a measure of mature granulocyte activity and MPO, granulocyte enzyme responsible for the cytotoxicity. Additionally we have investigated the plasma level of G-CSF which can increase in breast cancer. We tested 16 patients with I stage breast cancer and 15 patients with II stage breast cancer. Plasma samples were drawn before and 30 days after operation. The control group consisted of 10 healthy women. The plasma levels of sL-selectin, MPO and G-CSF were measured using breast cancer patients (I and II stage) before and after operation of sL-selection concentration was increased in comparison to the control group, but myeloperoxidase concentration was similar to the control group. These results suggest that levels of sL-selectin and MPO correlate with G-CSF concentration.
粒细胞集落刺激因子（G-CSF）是粒细胞集落形成的刺激因子。在体外和体内，G-CSF均可增强成熟细胞的功能。这方面的间接证据可能是参与吞噬作用和杀伤肿瘤细胞的粒细胞酶活性增加。本研究的目的是评估乳腺癌患者血浆中黏附分子——可溶性淋巴细胞选择素（sL-选择素）的水平，以此作为成熟粒细胞活性的指标，并评估髓过氧化物酶（MPO）的水平，MPO是负责细胞毒性的粒细胞酶。此外，我们还研究了乳腺癌患者血浆中可能升高的G-CSF水平。我们测试了16例I期乳腺癌患者和15例II期乳腺癌患者。在手术前和手术后30天采集血浆样本。对照组由10名健康女性组成。使用[具体检测方法未提及]测量乳腺癌患者（I期和II期）手术前后血浆中sL-选择素、MPO和G-CSF的水平。与对照组相比，sL-选择素浓度升高，但髓过氧化物酶浓度与对照组相似。这些结果表明，sL-选择素和MPO的水平与G-CSF浓度相关。
