Department of Organ Reconstruction, Institute for Frontier Medical Sciences, Kyoto University, Japan.
Biochem Biophys Res Commun. 2010 Mar 12;393(3):362-4. doi: 10.1016/j.bbrc.2009.12.152. Epub 2009 Dec 31.
Recent studies reported that bone marrow cavity offers a widely distributed and well-vascularized microenvironment which is a considerable implantation site for bioartificial pancreas (BAP). In this study, the in vivo performance of BAPs in bone marrow was further demonstrated in a spontaneous diabetes animal. Mouse insulinoma cells encapsulating in agarose gel were enclosed in a calcium phosphate cement chamber to create a BAP. Ten BAPs were implanted into the femur bone marrow cavity of a diabetic feline. The preprandial blood glucose level, 2 h glucose curve, serum C-peptide level and physiological conditions of the recipient were recorded perioperatively. Results showed that the cat still suffered from hyperglycemia postoperatively. However, the physiological conditions of feline were improved with an increase of serum C-peptide level. The peak point of 2 h glucose curve decreased from 400 to 165-290 mg/dl. The efficiency of exogenous insulin extended from 2 to 10-14 h postoperatively which reveals that the implanted BAPs had partial function. This case report revealed that BAPs implanted in the bone marrow cavity for the spontaneous diabetic is effective. The implanted BAPs provided therapeutic benefit despite sustained hyperglycemia. Further study shall be considered to improve the outcomes of BAPs transplantation.
最近的研究报告称，骨髓腔提供了一个广泛分布且血管丰富的微环境，这是生物人工胰腺（BAP）的一个相当理想的植入部位。在这项研究中，进一步在自发性糖尿病动物中证明了 BAP 在骨髓中的体内性能。将包封在琼脂糖凝胶中的小鼠胰岛素瘤细胞封装在磷酸钙水泥室内，以创建 BAP。将 10 个 BAP 植入糖尿病猫的股骨骨髓腔中。记录围手术期的餐前血糖水平、2 小时血糖曲线、血清 C 肽水平和受者的生理状况。结果表明，猫在手术后仍然患有高血糖症。然而，随着血清 C 肽水平的增加，猫的生理状况得到了改善。2 小时血糖曲线的峰值从 400 降至 165-290mg/dl。外源性胰岛素的效率从术后 2 小时延长至 10-14 小时，这表明植入的 BAP 具有部分功能。本病例报告显示，植入骨髓腔的 BAP 对自发性糖尿病是有效的。尽管持续存在高血糖症，但植入的 BAP 提供了治疗益处。应考虑进一步研究以改善 BAP 移植的结果。
