Division of Psychiatry and Neuroscience, Queen's University Belfast, Northern Ireland, United Kingdom.
Behav Brain Res. 2010 Apr 2;208(2):479-83. doi: 10.1016/j.bbr.2009.12.030. Epub 2009 Dec 31.
Tryptophan depletion techniques are effective in reducing central serotonergic function and have been used to investigate its role in mood and cognition. In the present study a tryptophan-free diet was fed to Lister-hooded male rats chronically for 21 days to investigate the effect of lowering central serotonin concentration on cognition using the novel object-recognition paradigm. Chronically tryptophan-depleted rats had impaired object-recognition memory; this was accompanied by a reduction in central serotonin of 40-50% in the hippocampus, frontal cortex and striatum. In a subsequent experiment, the atypical antipsychotic, risperidone (0.2 mg/kg), but not the typical antipsychotic, haloperidol (0.1 mg/kg), administered i.p. 30 min prior to the retention test, significantly attenuated the chronic tryptophan depletion impairment. These data show that chronic lowering of central serotonin is associated with impaired cognitive performance, and that this can be reversed by the atypical antipsychotic, risperidone.
色氨酸耗竭技术可有效降低中枢 5-羟色胺能功能，已被用于研究其在情绪和认知中的作用。在本研究中，通过给雄性 Lister-hooded 大鼠喂食不含色氨酸的饮食，慢性喂养 21 天，使用新物体识别范式研究降低中枢 5-羟色胺浓度对认知的影响。慢性色氨酸耗竭大鼠的物体识别记忆受损；这伴随着海马体、前额皮质和纹状体中 40-50%的中枢 5-羟色胺减少。在随后的实验中，非典型抗精神病药利培酮（0.2mg/kg），而非典型抗精神病药氟哌啶醇（0.1mg/kg），在保留测试前 30 分钟通过腹腔注射给药，显著减轻了慢性色氨酸耗竭损伤。这些数据表明，中枢 5-羟色胺的慢性降低与认知表现受损有关，而这种情况可以被非典型抗精神病药利培酮逆转。
