Baylor College of Medicine, One Baylor Plaza, MS: BCM 280, Houston, TX 77030, United States.
Vaccine. 2010 Feb 25;28(9):2076-9. doi: 10.1016/j.vaccine.2009.12.041. Epub 2009 Dec 29.
Antibody to the neuraminidase (NA) antigen of influenza viruses has been shown to correlate with immunity to influenza in humans and animal models. In a previous report, we showed that an inactivated influenza vaccine containing 60microg of the hemagglutinin (HA) of each strain induced significantly more serum anti-HA antibody among elderly persons than did the standard vaccine containing 15microg of the HA of each component. We developed a lectin-based assay for anti-NA antibody and used it to measure anti-NA antibody responses among subjects who had participated in that study. The high dosage vaccine contained eight times as much NA activity as the standard vaccine and induced a significantly higher frequency of antibody responses and higher mean postvaccination anti-NA titers to the N1 and N2 of the A/H1N1 and A/H3N2 viruses in the vaccines than did the standard vaccine. Ensuring an increased antibody response to the NA antigen in inactivated influenza virus vaccines should increase the protection against influenza. An increased quantity of the NA antigen in the vaccine will ensure an increased response.
抗流感病毒神经氨酸酶 (NA) 抗原的抗体已被证明与人类和动物模型对流感的免疫相关。在之前的一份报告中，我们表明，含有 60μg 每种毒株血凝素 (HA) 的灭活流感疫苗在老年人中诱导的血清抗-HA 抗体显著多于含有 15μg 每种成分 HA 的标准疫苗。我们开发了一种基于凝集素的抗-NA 抗体检测方法，并将其用于测量参与该研究的受试者的抗-NA 抗体反应。高剂量疫苗中含有标准疫苗八倍的 NA 活性，并且诱导针对疫苗中 A/H1N1 和 A/H3N2 病毒的 N1 和 N2 的抗体反应频率更高，平均接种后抗-NA 滴度更高。在灭活流感病毒疫苗中增加对 NA 抗原的抗体反应应该会增加对流感的保护。疫苗中增加 NA 抗原的数量将确保增加反应。
