Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, China.
J Chromatogr A. 2010 Feb 19;1217(8):1298-304. doi: 10.1016/j.chroma.2009.12.037. Epub 2009 Dec 22.
Macroporous cellulose beads (MCB) used as anion exchangers were successfully prepared from cellulose solution in ionic liquid by double emulsification followed by cross-linking and modification with diethylaminoethyl. The pore structure and properties of the MCB were investigated and the results were compared with homogeneous cellulose beads (HCB). The MCB in size of about 71 microm is characterized by two sets of pores, i.e., diffusion pores (10-20 nm) and macropores (800-2000 nm), determined by mercury porosimeter. In addition, the bed permeability and effective porosity for BSA of MCB-packed column are 58% and 25% higher than those of HCB-packed column, respectively. The adsorption properties of MCB were evaluated, and compared with HCB and commercial absorbent (Sepharose 6 Fast Flow, CSFF). It is found that the pore diffusivity of BSA in MCB is over 7.9 times higher than HCB, and 6.7 times higher than CSFF, respectively. While the equilibrium adsorption capacity (q(m)) of BSA on MCB is obviously lower than that on HCB and CSFF, the dynamic binding capacity (DBC) on MCB at 10% breakthrough reaches 47.7 mg/mL, higher than HCB (40.3mg/mL) and CSFF (46.2mg/mL) at flow rate of 360 cm/h. In addition, the MCB-packed column showed better column efficiency over the HCB packed one. Therefore, we demonstrated that the MCB possessed more advantages than other ones, like HCB and CSFF, and was expected as an ideal material for fast chromatography.
大孔纤维素珠（MCB）用作阴离子交换剂，是由纤维素在离子液体中的溶液通过双乳化作用，随后交联和二乙氨基乙基改性成功制备的。研究了 MCB 的孔结构和性能，并将结果与均相纤维素珠（HCB）进行了比较。MCB 的尺寸约为 71 微米，其特点是具有两组孔，即扩散孔（10-20nm）和大孔（800-2000nm），这是由压汞仪确定的。此外，MCB 填充柱的床渗透率和 BSA 的有效孔隙率分别比 HCB 填充柱高 58%和 25%。评估了 MCB 的吸附性能，并与 HCB 和商业吸附剂（Sepharose 6 Fast Flow，CSFF）进行了比较。结果发现，BSA 在 MCB 中的孔扩散系数比 HCB 高 7.9 倍，比 CSFF 高 6.7 倍。尽管 MCB 上 BSA 的平衡吸附容量（q(m)）明显低于 HCB 和 CSFF，但在 10%突破时，MCB 的动态结合容量（DBC）达到 47.7mg/mL，高于 HCB（40.3mg/mL）和 CSFF（46.2mg/mL）在 360cm/h 的流速下。此外，MCB 填充柱的柱效明显优于 HCB 填充柱。因此，我们证明了 MCB 比 HCB 和 CSFF 等其他材料具有更多的优势，有望成为快速色谱的理想材料。
