Du Kaifeng
Department of Pharmaceutical & Biological Engineering, School of Chemical Engineering, Sichuan University, Chengdu 610065, PR China.
J Chromatogr A. 2017 Apr 21;1494:40-45. doi: 10.1016/j.chroma.2017.03.004. Epub 2017 Mar 6.
Macroporous cellulose monolith as chromatographic support was successfully fabricated from an ionic liquid dissolved cellulose solution by an emulsification method and followed by the cross-linking reaction and DEAE modification. With the physical characterization, the cellulose monolith featured by both the interconnected macropores in range of 0.5-2.5μm and the diffusion pores centered at about 10nm. Given the bimodal pore system, the monolith possessed the specific surface area of 36.4mg and the column permeability of about 7.45×10m. After the DEAE modification, the anion cellulose monolith was evaluated for its chromatography performances. It demonstrated that the static and dynamic adsorption capacity of BSA reached about 66.7mgmL and 43.9mgmL at 10% breakthrough point, respectively. The results were comparable to other chromatographic adsorbent. In addition, the proteins mixture with different pI was well separated at high flow velocity (611.0cmh) and high protein recovery (over 97%), proving the macroporous cellulose monolith had excellent separation performance. In this way, the prepared cellulose monolith with bimodal pores system is expected for the potential application in high-speed chromatography.
通过乳化法，以离子液体溶解的纤维素溶液为原料，经交联反应和DEAE改性，成功制备了大孔纤维素整体柱作为色谱载体。通过物理表征可知，该纤维素整体柱具有0.5 - 2.5μm范围内相互连通的大孔以及以10nm左右为中心的扩散孔。鉴于其双峰孔体系，该整体柱的比表面积为36.4mg，柱渗透率约为7.45×10m。DEAE改性后，对阴离子纤维素整体柱的色谱性能进行了评估。结果表明，在10%穿透点时，BSA的静态和动态吸附容量分别达到约66.7mg/mL和43.9mg/mL。这些结果与其他色谱吸附剂相当。此外，不同pI的蛋白质混合物在高流速（611.0cm/h）和高蛋白回收率（超过97%）下得到了很好的分离，证明该大孔纤维素整体柱具有优异的分离性能。通过这种方式，制备的具有双峰孔体系的纤维素整体柱有望在高速色谱中得到潜在应用。
