Department of Chemical Engineering, University of Washington, Seattle, WA 98195, USA.
Biomaterials. 2010 Apr;31(10):2919-25. doi: 10.1016/j.biomaterials.2009.12.038. Epub 2010 Jan 4.
In this work, we report a tunable mixed-charge copolymer surface containing positively charged quaternary amine monomers ([2-(acryloyloxy)ethyl] trimethyl ammonium chloride, TMA) and negatively-charged carboxylic acid monomers (2-carboxy ethyl acrylate, CAA). The non-fouling properties of this copolymer coating depend on environmental pH. The surface has charge neutrality under neutral and basic conditions, and is positively charged under acidic conditions due to the protonation of the carboxylic acid group. This transition in surface charge with respect to pH allows the surface to be switched from bacteria-adhesive to bacteria-resistant. We demonstrate that the bacteria adhered to the surface under acidic conditions can be easily released as bulk pH increases. This tunable surface can be used to collect a contaminant and then be externally stimulated to release the contaminant, to allow for analysis of its composition. Its bacteria attraction and release property makes it very promising for decontamination and biomedical applications.
在这项工作中，我们报告了一种可调谐的混合电荷共聚物表面，其中包含带正电荷的季铵单体[2-（丙烯酰氧基）乙基]三甲基氯化铵（TMA）和带负电荷的羧酸单体（2-羧乙基丙烯酸酯，CAA）。该共聚物涂层的非粘性特性取决于环境 pH 值。在中性和碱性条件下，表面带电荷中性，由于羧酸基团的质子化，在酸性条件下带正电荷。这种表面电荷随 pH 值的变化允许表面从细菌粘附状态切换到细菌抵抗状态。我们证明，在酸性条件下粘附在表面上的细菌可以很容易地在 bulk pH 值增加时释放。这种可调谐表面可用于收集污染物，然后通过外部刺激释放污染物，以允许分析其成分。其细菌吸引力和释放特性使其在去污和生物医学应用中具有很大的应用前景。
