Department of Materials, Imperial College London, Exhibition Road, London SW7 2AZ, UK; Natural History Museum, Mineralogy, Cromwell Road, London SW75BD, UK.
Biomaterials. 2010 Apr;31(10):2806-15. doi: 10.1016/j.biomaterials.2009.12.045. Epub 2010 Jan 4.
Poly(3-hydroxybutyrate) (P(3HB)) foams exhibiting highly interconnected porosity (85% porosity) were prepared using a unique combination of solvent casting and particulate leaching techniques by employing commercially available sugar cubes as porogen. Bioactive glass (BG) particles of 45S5 Bioglass grade were introduced in the scaffold microstructure, both in micrometer ((m-BG), <5 microm) and nanometer ((n-BG), 30 nm) sizes. The in vitro bioactivity of the P(3HB)/BG foams was confirmed within 10 days of immersion in simulated body fluid and the foams showed high level of protein adsorption. The foams interconnected porous microstructure proved to be suitable for MG-63 osteoblast cell attachment and proliferation. The foams implanted in rats as subcutaneous implants resulted in a non-toxic and foreign body response after one week of implantation. In addition to showing bioactivity and biocompatibility, the P(3HB)/BG composite foams also exhibited bactericidal properties, which was tested on the growth of Staphylococcus aureus. An attempt was made at developing multifunctional scaffolds by incorporating, in addition to BG, selected concentrations of Vitamin E or/and carbon nanotubes. P(3HB) scaffolds with multifunctionalities (viz. bactericidal, bioactive, electrically conductive, antioxidative behaviour) were thus produced, which paves the way for next generation of advanced scaffolds for bone tissue engineering.
聚（3-羟基丁酸酯）（P（3HB））泡沫具有高度互连的孔隙率（85%的孔隙率），是通过使用市售的糖块作为成孔剂，采用独特的溶剂浇铸和颗粒浸出技术制备的。生物活性玻璃（BG）颗粒的 45S5 Bioglass 级被引入支架微结构中，包括微米级（（m-BG），<5 微米）和纳米级（（n-BG），30nm）。P（3HB）/BG 泡沫在模拟体液中浸泡 10 天内即可确认其生物活性，并且泡沫表现出高水平的蛋白质吸附。泡沫的互穿多孔微观结构被证明适合 MG-63 成骨细胞的附着和增殖。泡沫作为皮下植入物植入大鼠体内，在植入一周后表现出非毒性和异物反应。除了表现出生物活性和生物相容性外，P（3HB）/BG 复合泡沫还表现出杀菌性能，这在金黄色葡萄球菌的生长测试中得到了验证。通过在 BG 中加入选定浓度的维生素 E 或/和碳纳米管，尝试开发多功能支架。具有多功能性（即杀菌、生物活性、导电性、抗氧化性）的 P（3HB）支架得以生产，为下一代骨组织工程的先进支架铺平了道路。
