Djondjurov L, Tsvetkov S, Ivanova E
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Exp Cell Res. 1991 Apr;193(2):291-6. doi: 10.1016/0014-4827(91)90099-g.
By using various approaches, we have observed in cycling mouse erythroleukemia cells a characteristic sequential deposition of newly synthesized histones. Control experiments confirmed that this deposition is replicative and is not associated with a process of histone replacement. Interestingly, each of the newly synthesized variants of histones H3, H2B, and H2A was found to deposit simultaneously. This observation indicates that the mechanisms governing the nucleosome assembly do not discriminate among the variants and thus supports the idea that they have equal biological significance. In contrast, a different mode of deposition was found for the regular subtypes H1A and H1B, suggesting a structural and functional difference between these two histones.
通过使用各种方法，我们在循环的小鼠红白血病细胞中观察到新合成组蛋白的特征性顺序沉积。对照实验证实这种沉积是复制性的，并且与组蛋白置换过程无关。有趣的是，发现组蛋白H3、H2B和H2A的每个新合成变体同时沉积。这一观察结果表明，控制核小体组装的机制不会区分这些变体，因此支持它们具有同等生物学意义的观点。相比之下，发现常规亚型H1A和H1B的沉积模式不同，这表明这两种组蛋白在结构和功能上存在差异。
