体外复制DNA上的染色质组装。
Chromatin assembly on replicating DNA in vitro.
作者信息
Almouzni G, Clark D J, Méchali M, Wolffe A P
机构信息
Laboratory of Molecular Biology, NIDDK, National Institutes of Health, Bethesda, MD 20892.
出版信息
Nucleic Acids Res. 1990 Oct 11;18(19):5767-74. doi: 10.1093/nar/18.19.5767.
Replicating single-stranded DNA is preferentially assembled into chromatin in Xenopus egg extracts relative to non-replicating double-stranded DNA. We have examined the molecular basis of this phenomenon. Single-stranded DNA itself is not a favored template for nucleosome assembly in comparison to double-stranded DNA. Complementary strand synthesis is required for the rapid assembly of nucleosomes. We present evidence that the assembly of chromatin on replicating DNA is a two step phenomenon. The first step involves the replication of DNA and the assembly of an intermediate structure, the second step involves the sequestration of histones H2A/H2B onto DNA. Histones H2A/H2B are preferentially sequestered onto replicated DNA in comparison to non-replicated DNA incubated in the extract.
相对于非复制性双链DNA，在非洲爪蟾卵提取物中，复制性单链DNA优先组装成染色质。我们已经研究了这种现象的分子基础。与双链DNA相比，单链DNA本身并不是核小体组装的理想模板。核小体的快速组装需要互补链的合成。我们提供的证据表明，复制性DNA上染色质的组装是一个两步过程。第一步涉及DNA的复制和中间结构的组装，第二步涉及组蛋白H2A/H2B与DNA的结合。与提取物中孵育的未复制DNA相比，组蛋白H2A/H2B优先与复制后的DNA结合。
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