Department of Radiology, Daejeon St. Mary's Hospital, College of Medicine, The Catholic University of Korea, Daejeon, Korea.
Yonsei Med J. 2010 Jan;51(1):69-76. doi: 10.3349/ymj.2010.51.1.69. Epub 2009 Dec 29.
Stem cell transplantation is expected to have good effects in the treatment of myocardial infarction (MI). We tested the effect of the transplantation of human adipose-derived cells (ASCs) in Sprague-Dawley (SD) rats with myocardial infarctions.
ASCs were isolated from the waste of elective abdominal surgery. The MI model was set up in SD rats by permanent ligation of the left anterior descending coronary artery. One week after MI, either 1 x 10(6) ASCs or an equal volume of phosphate-buffered saline (PBS) was injected into the infarct zone. Cardiac function was assessed by echocardiography, 1 day, 1 week, 2 weeks, and 4 weeks after treatment. Four weeks after transplantation, immunohistochemistry was performed.
Left ventricular function, including fractional shortening (FS), and ejection fraction (EF) showed a significant improvement in the ASCs transplantation group compared to the PBS group 4 weeks after treatment (p < 0.05). The anterior wall thickness of the left ventricle was significantly thicker in the ASCs transplantation group compared to the PBS group (p < 0.01). Multiple troponin T staining, and irregular, small amounts of connexin 43 expression also was observed in the ASCs transplantation group. Infarcted myocardium showed higher capillary density in the ASCs transplantation group than in the PBS injected group (p < 0.01).
This study provides encouraging evidence that transplantation of ASCs can improve cardiac function of infarct myocardium in rat models with a limitation of cardiac remodeling, improved wall thickness, and increased neovascularization.
干细胞移植有望在治疗心肌梗死（MI）方面产生良好效果。我们测试了将人脂肪来源细胞（ASCs）移植到心肌梗死的 Sprague-Dawley（SD）大鼠中的效果。
从择期腹部手术的废物中分离出 ASCs。通过永久性结扎左前降支冠状动脉在 SD 大鼠中建立 MI 模型。在 MI 后 1 周，将 1x10(6)个 ASCs 或等量的磷酸盐缓冲盐水（PBS）注射到梗死区。治疗后 1 天、1 周、2 周和 4 周，通过超声心动图评估心功能。移植后 4 周，进行免疫组织化学染色。
与 PBS 组相比，ASCs 移植组左心室功能（包括缩短分数（FS）和射血分数（EF））在治疗 4 周后显著改善（p<0.05）。ASCs 移植组的左心室前壁厚度明显比 PBS 组厚（p<0.01）。ASCs 移植组还观察到多个肌钙蛋白 T 染色和连接蛋白 43 的不规则、少量表达。与 PBS 注射组相比，ASCs 移植组梗死心肌的毛细血管密度更高（p<0.01）。
这项研究提供了令人鼓舞的证据，表明 ASCs 移植可以改善大鼠模型中梗死心肌的心脏功能，限制心脏重构，增加壁厚度，并增加新生血管形成。
