Oh Shin-Taek, Cho Baik-Kee, Schramme Anja, Gutwein Paul, Tilgen Wolfgang, Reichrath Jörg
Department of Dermatology; The Saarland University Hospital; Homburg/Saar, Germany.
Dermatoendocrinol. 2009 Jan;1(1):46-53. doi: 10.4161/derm.1.1.7497.
ADAM proteases play important roles in processes of development and differentiation. However, no report has been found in the literature addressing the expression and function of ADAM proteases during hair cycling.
Cytoplasmic expression pattern of ADAM 10, 12 was similar between normal epidermis and hair infundibulum. In addition, cytoplasmic expression of ADAM 10 was observed in the hair bulb keratinocytes and fibroblasts of dermal papilla in anagen I-III hair follicles. In contrast, decreased ADAM 10 expression was observed in the hair matrix keratinocytes as compared to the hair bulb keratinocytes in anagen I-III hair follicles. Interestingly, ADAM 10 immunoreactivity was expressed weakly in the lower portion of outer root sheath (ORS) of anagen VI hair follicles, and strong ADAM 10 expression was detected in the ORS of catagen and telogen hair follicles. By contrast, ADAM 12 expression was not detected in the hair bulb keratinocytes of anagen I-III hair follicles. ADAM 12 immunoreactivity firstly appeared in the inner root sheath ( IRS ) of anagen IV-V hair follicles and was down-regulated in the IRS and hair cortex and medulla of catagen hair follicles, Strong ADAM 12 immunoreactivity was observed in the ORS of catagen and telogen hair follicles.
Samples of normal human skin (n = 30) were used. Immunohistochemical analysis was performed using ADAM 10, 12 specific polyclonal antibodies and a sensitive streptavidin-peroxidase technique.
Our study demonstrates a comparable staining pattern of decreased ADAM 10 immunoreactivity in hair matrix keratinocytes and the basal cell layer of normal epidermis and hair infundibulum. Expression of ADAM 10 in dermal papilla cells may imply a role in the induction and development of anagen hair follicles. In addition, expression of ADAM 10 in the ORS and hair bulb assume the involvment of ADAM 10 in the downward migration of anagen hair follicles. Furthermore ADAM 12 expression in the IRS may indicate a role in the differentiation of anagen hair follicles. Downregulation of ADAM 12 upon the onset of catagen hair stage suggests that ADAM 12 may play an important role of ADAM 12 in the apoptosis of hair follicle keratinocytes. In summary our findings suggest that ADAM 10 and 12 may be of importance for the regulation of hair cycling.
ADAM蛋白酶在发育和分化过程中发挥重要作用。然而，尚未在文献中发现有关ADAM蛋白酶在毛发周期中的表达和功能的报道。
ADAM 10、12在正常表皮和毛囊漏斗部的细胞质表达模式相似。此外，在生长期I-III毛囊的毛球角质形成细胞和毛乳头成纤维细胞中观察到ADAM 10的细胞质表达。相比之下，与生长期I-III毛囊的毛球角质形成细胞相比，在毛基质角质形成细胞中观察到ADAM 10表达降低。有趣的是，ADAM 10免疫反应性在生长期VI毛囊外根鞘（ORS）的下部表达较弱，而在退行期和休止期毛囊的ORS中检测到强ADAM 10表达。相比之下，在生长期I-III毛囊的毛球角质形成细胞中未检测到ADAM 12表达。ADAM 12免疫反应性首先出现在生长期IV-V毛囊的内根鞘（IRS）中，并在退行期毛囊的IRS、毛皮质和髓质中下调，在退行期和休止期毛囊的ORS中观察到强ADAM 12免疫反应性。
使用正常人皮肤样本（n = 30）。使用ADAM 10、12特异性多克隆抗体和灵敏的链霉亲和素-过氧化物酶技术进行免疫组织化学分析。
我们的研究表明，在毛基质角质形成细胞以及正常表皮和毛囊漏斗部的基底细胞层中，ADAM 10免疫反应性降低的染色模式具有可比性。ADAM 10在毛乳头细胞中的表达可能暗示其在生长期毛囊的诱导和发育中起作用。此外，ADAM 10在ORS和毛球中的表达表明ADAM 10参与了生长期毛囊的向下迁移。此外，ADAM 12在IRS中的表达可能表明其在生长期毛囊分化中起作用。在退行期毛发阶段开始时ADAM 12的下调表明ADAM 12可能在毛囊角质形成细胞的凋亡中起重要作用。总之，我们的研究结果表明ADAM 10和12可能对毛发周期的调节很重要。
