生殖相关ADAM基因家族中的表型补偿：以ADAM27基因敲除小鼠为例的研究
Phenotype Compensation in Reproductive ADAM Gene Family: A Case Study with ADAM27 Knockout Mouse.
作者信息
Khezri Jafar, Hasheni Ehsan, Shams Ara Mehdi, Rahim Tayefe Aidin, Heidari Farid
机构信息
Department of Animal Biotechnology, Faculty of Agriculture Biotechnology, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
出版信息
Iran J Biotechnol. 2022 Oct 1;20(4):e2902. doi: 10.30498/ijb.2022.250175.2902. eCollection 2022 Oct.
BACKGROUND
A disintegrin and metalloproteinase (ADAM) cell surface proteins are expressed in different cells and are involved in biological processes such as cell-cell interactions, cell differentiation, sperm attachment and fertilization. A significant number of ADAM isoforms are expressed in the reproductive tracts of male mice and other mammals, which shows the importance of this gene family in reproduction.
OBJECTIVES
The role of ADAM27 protein in reproduction was investigated.
MATERIALS AND METHODS
ADAM27 knock-out mutant mice were generated using the blastocyst microinjection technique. The knock-out mice were analyzed genetically and phenotypically to discover any abnormalities.
RESULTS
The results of this study revealed that the homozygote mutant male mice were fertile and showed no significant differences compared to wild-type male mice. A histological exam, sperm analysis and in-vitro fertilization experiments showed no statistical differences.
CONCLUSIONS
We can conclude that the role of deficient ADAM27 protein is probably compensated mainly by other ADAM isoforms which are expressed in the reproductive system.
背景
解整合素金属蛋白酶（ADAM）细胞表面蛋白在不同细胞中表达，并参与细胞间相互作用、细胞分化、精子附着和受精等生物学过程。大量ADAM亚型在雄性小鼠和其他哺乳动物的生殖道中表达，这表明该基因家族在生殖中的重要性。
目的
研究ADAM27蛋白在生殖中的作用。
材料与方法
利用囊胚显微注射技术构建ADAM27基因敲除突变小鼠。对基因敲除小鼠进行遗传和表型分析，以发现任何异常情况。
结果
本研究结果显示，纯合子突变雄性小鼠可育，与野生型雄性小鼠相比无显著差异。组织学检查、精子分析和体外受精实验均无统计学差异。
结论
我们可以得出结论，ADAM27蛋白缺乏的作用可能主要由生殖系统中表达的其他ADAM亚型来补偿。
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