同时用高效薄层色谱法测定雷贝拉唑和盐酸伊托必利复方制剂中二者的含量。
Simultaneous HPTLC Determination of Rabeprazole and Itopride Hydrochloride From Their Combined Dosage Form.
作者信息
Suganthi A, John Sofiya, Ravi T K
机构信息
Department of Pharmaceutical Analysis, College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore-641 044, India.
出版信息
Indian J Pharm Sci. 2008 May-Jun;70(3):366-8. doi: 10.4103/0250-474X.43004.
A simple, precise, sensitive, rapid and reproducible HPTLC method for the simultaneous estimation of the rabeprazole and itopride hydrochloride in tablets was developed and validated. This method involves separation of the components by TLC on precoated silica gel G60F254 plate with solvent system of n-butanol, toluene and ammonia (8.5:0.5:1 v/v/v) and detection was carried out densitometrically using a UV detector at 288 nm in absorbance mode. This system was found to give compact spots for rabeprazole (Rf value of 0.23 0.02) and for itopride hydrochloride (Rf value of 0.75+/-0.02). Linearity was found to be in the range of 40-200 ng/spot and 300-1500 ng/spot for rabeprazole and itopride hydrochloride. The limit of detection and limit of quantification for rabeprazole were 10 and 20 ng/spot and for itopride hydrochloride were 50 and 100 ng/spot, respectively. The method was found to be beneficial for the routine analysis of combined dosage form.
建立并验证了一种用于同时测定片剂中雷贝拉唑和盐酸伊托必利的简单、精确、灵敏、快速且可重现的高效薄层色谱法。该方法采用在预涂硅胶G60F254板上进行薄层色谱分离，溶剂系统为正丁醇、甲苯和氨（8.5:0.5:1 v/v/v），并使用紫外检测器在288 nm波长下以吸光度模式进行光密度测定。结果发现，该系统对雷贝拉唑产生紧密斑点（比移值为0.23±0.02），对盐酸伊托必利产生紧密斑点（比移值为0.75±0.02）。雷贝拉唑和盐酸伊托必利的线性范围分别为40 - 200 ng/斑点和300 - 1500 ng/斑点。雷贝拉唑的检测限和定量限分别为1 ng/斑点和20 ng/斑点，盐酸伊托必利的检测限和定量限分别为50 ng/斑点和100 ng/斑点。该方法被认为对复方制剂的常规分析有益。
Zotero 插件