Tian D F, Tian X L, Tian T, Wang Z Y, Mo F K
Department of Pharmacy, School of Pharmacy, Shenyang Pharmaceutical University, 110016 Shenyang, China.
Indian J Pharm Sci. 2008 May-Jun;70(3):372-4. doi: 10.4103/0250-474X.43006.
A simple, reproducible and efficient reverse phase high performance liquid chromatographic method was developed for simultaneous determination of valsartan and hydrochlorothiazide in tablets. A column having 200 x 4.6 mm i.d. in isocratic mode with mobile phase containing methanol:acetonitrile:water:isopropylalcohol (22:18:68:2; adjusted to pH 8.0 using triethylamine; v/v) was used. The flow rate was 1.0 ml/min and effluent was monitored at 270 nm. The retention time (min) and linearity range (mug/ml) for valsartan and hydrochlorothiazide were (3.42, 8.43) and (5-150, 78-234), respectively. The developed method was found to be accurate, precise and selective for simultaneous determination of valsartan and hydrochlorothiazide in tablets.
建立了一种简单、可重现且高效的反相高效液相色谱法，用于同时测定片剂中的缬沙坦和氢氯噻嗪。使用内径为200×4.6 mm的色谱柱，在等度模式下，流动相为甲醇：乙腈：水：异丙醇（22:18:68:2；用三乙胺调节至pH 8.0；v/v）。流速为1.0 ml/min，在270 nm处监测流出物。缬沙坦和氢氯噻嗪的保留时间（分钟）和线性范围（μg/ml）分别为（3.42，8.43）和（5 - 150，78 - 234）。所建立的方法被发现对于同时测定片剂中的缬沙坦和氢氯噻嗪是准确、精密且具有选择性的。
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