Eppley Institute for Research in Cancer and Allied Diseases, University of Nebraska Medical Center, Omaha, Nebraska, USA.
Zebrafish. 2009 Dec;6(4):329-37. doi: 10.1089/zeb.2009.0626.
This review highlights the current techniques used to generate transgenic mouse models of cancer, with an emphasis on recent advances in the use of ubiquitous promoters, models that use Cre-loxP and Flip-FRT recombinase technology, inducible systems, RNAi to target genes, and transposon mutagenesis. A concluding section discusses new imaging systems that visualize tumor progression and the microenvironment in vivo. In this review, these techniques and strategies used in mouse models of cancer are highlighted, as they are pertinent and relevant to the development of zebrafish models of cancer.
本文重点介绍了目前用于生成癌症转基因小鼠模型的技术，强调了广泛使用启动子、利用 Cre-loxP 和 Flip-FRT 重组酶技术、诱导系统、RNAi 靶向基因以及转座子诱变的最新进展。最后一节讨论了可用于可视化肿瘤进展和体内微环境的新型成像系统。在本文中，突出强调了这些在癌症小鼠模型中使用的技术和策略，因为它们与癌症斑马鱼模型的开发密切相关。
