Reproductive Immunology, IDEHU (CONICET) - FFyB, University of Buenos Aires, Buenos Aires, Argentina.
Am J Reprod Immunol. 2010 Mar 1;63(3):193-9. doi: 10.1111/j.1600-0897.2009.00778.x. Epub 2009 Dec 29.
Trophoblast invasion is a temporally and locally restricted process, which regulates implantation and oxygen arrival to the embryo through the dialog with spiral artery endothelium. Trophoblast factors with angiogenic potential are activated by hypoxia. Their capacities to induce proliferation, migration, and invasion of trophoblastic cells have been investigated.
The expression of interleukin (IL)-6, CD126, CD130, vascular endothelial growth factor (VEGF), and hypoxia inducible factor-1alpha (HIF-1alpha) has been silenced in JEG-3 choriocarcinoma cells by using siRNA. Silencing efficacy has been assessed by ELISA, PCR or Western blotting. Proliferation has been measured by flow cytometry, migration by a transwell assay, and invasion by a Matrigel assay.
Proliferation was significantly reduced by silencing of CD126 or CD130, migration by silencing of IL-6, VEGF, or HIF-1alpha, and invasion by silencing of IL-6 and HIF-1alpha.
The expression of IL-6, VEGF, and HIF-1alpha in trophoblastic cells is involved in the control of trophoblast invasion and migration.
滋养细胞浸润是一个时间和空间受限的过程，它通过与螺旋动脉内皮的对话来调节胚胎的着床和氧气供应。具有血管生成潜力的滋养细胞因子在缺氧的情况下被激活。已经研究了它们诱导滋养细胞增殖、迁移和浸润的能力。
采用 siRNA 沉默 JEG-3 绒癌细胞中白细胞介素 (IL)-6、CD126、CD130、血管内皮生长因子 (VEGF) 和缺氧诱导因子-1alpha (HIF-1alpha) 的表达。通过 ELISA、PCR 或 Western blot 评估沉默效果。通过流式细胞术测量增殖，通过 Transwell 测定迁移，通过 Matrigel 测定侵袭。
沉默 CD126 或 CD130 显著降低增殖，沉默 IL-6、VEGF 或 HIF-1alpha 降低迁移，沉默 IL-6 和 HIF-1alpha 降低侵袭。
滋养细胞中 IL-6、VEGF 和 HIF-1alpha 的表达参与了滋养细胞浸润和迁移的调控。
