Department of Biomedical Engineering, College of Medicine, Kyung Hee University, Dongdaemun-gu, Seoul, Republic of Korea.
Rheumatol Int. 2011 May;31(5):595-603. doi: 10.1007/s00296-009-1333-5. Epub 2010 Jan 5.
The exact mechanism of rheumatoid arthritis (RA) is unclear, but a combination of genetic, environmental and hormonal factors is thought to be involved. This study examined the progressive arthritic reaction of murine type II collagen-induced arthritis (CIA), a representative animal model of RA. Arthritic reactions, including inflammation and bone erosion were examined using an objective non-invasive method. Two scoring systems were used to evaluate changes in cutaneous inflammation and bone erosion during RA progression. The severity of inflammation was evaluated by visual scoring of erythema and edema, while the degree of bone erosion was quantified by macroradiographical erosion analysis of specific bones. A significant difference was observed in both visual (P = 0.0001, n = 7) and radiographic (P < 0.0001, n = 7) examinations for the RA group as compared to the control. The relationship between inflammatory change and erosive change in bone showed a significant positive correlation, r = 0.9550 (P < 0.0001, n = 7). The overall rate of asymmetry was 25.23% in both fore- and hindpaws. The results generated from these experiments show that murine CIA is a promising model for elucidating the mechanism of RA. In addition, the results of this study may be used for monitoring RA progression as well as screening therapy efficacy in the joint pathology.
类风湿关节炎（RA）的确切机制尚不清楚，但遗传、环境和激素因素的综合作用被认为与之相关。本研究检查了Ⅱ型胶原诱导关节炎（CIA）的进行性关节炎反应，这是一种 RA 的代表性动物模型。采用客观的非侵入性方法检查关节炎反应，包括炎症和骨侵蚀。使用两种评分系统评估 RA 进展过程中皮肤炎症和骨侵蚀的变化。通过对特定骨骼的宏观放射性侵蚀分析，对炎症的严重程度进行视觉评分，评估红斑和水肿；对骨侵蚀的程度进行定量评估。与对照组相比，RA 组在视觉（P = 0.0001，n = 7）和放射学（P < 0.0001，n = 7）检查中均有显著差异。炎症变化与骨侵蚀变化之间存在显著正相关，r = 0.9550（P < 0.0001，n = 7）。前肢和后肢的不对称总体发生率分别为 25.23%。这些实验结果表明，鼠 CIA 是阐明 RA 发病机制的一种很有前途的模型。此外，本研究的结果可用于监测 RA 进展以及关节病理学中的治疗效果筛选。
