Department of Biomedical Engineering & Healthcare Industry Research Institute, College of Medicine, Kyung Hee University, 1 Hoegi-dong, Dongdaemun-gu, Seoul, 130-701, Republic of Korea.
Inflamm Res. 2011 May;60(5):447-56. doi: 10.1007/s00011-010-0287-0. Epub 2010 Dec 12.
This study presents a method for non-invasive screening of progressive arthritic reactions in a representative rheumatoid arthritis (RA) animal model of Type II collagen-induced arthritis (CIA).
Seventeen male DBA/1J mice (9 weeks old) were used for the control group (seven) and the CIA group (ten).
The CIA model was induced by the injection of a Type II collagen and incomplete Freund's adjuvant emulsion.
Arthritic reactions including joint space narrowing (JSN), bone erosion, inflammation, and physiological defects were observed using five non-invasive methods. The degrees of JSN and bone erosion were quantified using macro-radiographs of specific joints and bones, while the severity of inflammation was evaluated by changes in paw volume and visual scoring of edema and erythema.
Significant differences were observed in JSN (≤48.29%, p < 0.0001), bone erosion (78.30%, p < 0.0001), grip strength (p < 0.05), and inflammation (91.67% in edema and 194.12% increase in paw volume, p < 0.001) between the CIA group and the controls. The relationships between factors exhibited significant positive correlations (R ≥ 0.9229, p < 0.05) between radiographic parameters, with symmetries of ≥0.9062 (p < 0.05) for JSN and ≥ 0.8575 (p < 0.001) for bone erosion, 0.8770 (p < 0.0001) for inflammatory parameters, and ≥0.9527 (p < 0.0001) for radiographic and non-radiographic parameters.
Our non-invasive radiographic and non-radiographic methods of documentation were both acceptable for screening joint defects during RA progression. In addition, the results of this study may be useful for monitoring the progression of joint pathology in RA diseases.
本研究提出了一种方法，用于在 II 型胶原诱导关节炎（CIA）的代表性类风湿关节炎（RA）动物模型中进行非侵入性的进行性关节炎反应筛查。
17 只 9 周龄雄性 DBA/1J 小鼠（7 只为对照组，10 只为 CIA 组）用于本研究。
通过注射 II 型胶原和不完全弗氏佐剂乳剂诱导 CIA 模型。
使用五种非侵入性方法观察关节间隙狭窄（JSN）、骨侵蚀、炎症和生理缺陷等关节炎反应。通过特定关节和骨骼的宏观射线照片定量 JSN 和骨侵蚀的程度，通过爪体积的变化和肿胀和红斑的视觉评分评估炎症的严重程度。
CIA 组与对照组在 JSN（≤48.29%，p<0.0001）、骨侵蚀（78.30%，p<0.0001）、握力（p<0.05）和炎症（肿胀增加 194.12%，p<0.001）方面存在显著差异。影像学参数之间的关系呈显著正相关（R≥0.9229，p<0.05），其中 JSN 的对称性≥0.9062（p<0.05），骨侵蚀的对称性≥0.8575（p<0.001），炎症参数的对称性为 0.8770（p<0.0001），影像学和非影像学参数的对称性≥0.9527（p<0.0001）。
我们的非侵入性影像学和非影像学记录方法均可用于筛查 RA 进展过程中的关节缺陷。此外，本研究的结果可能有助于监测 RA 疾病中关节病理学的进展。
