Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, GA 30332, USA.
Wiley Interdiscip Rev Nanomed Nanobiotechnol. 2010 Jan-Feb;2(1):11-9. doi: 10.1002/wnan.52.
Accurately imaging endogenous or non-engineered RNA in live cells is not an easy task. Ideally, a probe and imaging strategy will have the following properties: (1) functional probes will be delivered to the desired cellular compartment, (2) they will achieve the correct level of affinity to bind target RNA efficiently but not inhibit their function, (3) be sensitive enough to allow for the accurate detection of the cellular RNA population, and (4) allow for the tracking of RNA through biogenesis, transport, translation, and degradation pathways. In this review, the capabilities of current nucleic acid-based probes and strategies used to image native RNA are discussed and analyzed, and probe and strategy recommendations for new users are given. The review is concluded by addressing topics for future research, all in the hope of achieving the ideal RNA imaging probe and strategy.
准确地对活细胞内的内源性或非工程 RNA 进行成像并非易事。理想情况下，探针和成像策略应具有以下特性：（1）功能探针将递送到所需的细胞区室，（2）它们将达到与靶 RNA 有效结合的正确亲和力水平，但不会抑制其功能，（3）足够灵敏，以允许准确检测细胞 RNA 群体，以及（4）允许通过生物发生、运输、翻译和降解途径跟踪 RNA。在这篇综述中，讨论和分析了当前用于成像天然 RNA 的基于核酸的探针和策略的能力，并为新用户提供了探针和策略建议。最后通过解决未来研究的主题来结束综述，所有这些都是为了实现理想的 RNA 成像探针和策略。
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