Yongmanitchai W, Ward O P
Department of Biology, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1
J Gen Microbiol. 1993 Mar;139(3):465-72. doi: 10.1099/00221287-139-3-465.
Each of the four main polar lipids from Phaeodactylum tricornutum UTEX 640, monogalactyosyldiacylglycerol(MGDG), digalactosyldiacylglycerol (DGDG), sulphoquinovosyldiacylglycerol (SQDG) and phosphatidylcholine(PC), was separated into its molecular species by reverse-phase HPLC, and the positional distribution of fatty acids in each species was determined. Numbers of separated peaks in each lipid class were: MGDG, 10; DGDG,7; SQDG, 10; and PC, 11. Eicosapentaenoic acid (EPA) was present in 20 of the 45 molecular species but predominated in the MGDG and DGDG classes, where 13 of the proposed structures contained EPA. EPA was always located in the sn-1 position except in two lipid species, MG5 and PC2, where it was present at both the sn-1 and sn-2 locations. The predominant polar lipid molecular species found in P. tricornutum UTEX 640 were(mg total fatty acids per g dry weight of biomass) 20:5-16:4-MG, 41-2; 20:5-16:1-DG, 21.0; 16:1-16:1-SQ,18-2; and 20:5-16: 1-MG, 18-0. [Structure indicates fatty acid at sn-1 position - fatty acid at sn-2 position -carbohydrate component at sn-3 position of the glycerol molecule.
20: 5, EPA; 16: 4,hexadecatetraenoic acid ; 16 : 1, palmitoleic acid ; MG, monogalactose ; DG, digalactose ; SQ, sulphoquinovose.]
三角褐指藻UTEX 640的四种主要极性脂，单半乳糖基二酰基甘油（MGDG）、双半乳糖基二酰基甘油（DGDG）、磺基喹喔啉基二酰基甘油（SQDG）和磷脂酰胆碱（PC），通过反相高效液相色谱法分离成其分子种类，并测定每种种类中脂肪酸的位置分布。每个脂质类别的分离峰数为：MGDG，10个；DGDG，7个；SQDG，10个；PC，11个。二十碳五烯酸（EPA）存在于45种分子种类中的20种中，但在MGDG和DGDG类别中占主导地位，其中13种提议的结构含有EPA。EPA总是位于sn-1位置，除了两种脂质种类MG5和PC2，其中它同时存在于sn-1和sn-2位置。在三角褐指藻UTEX 640中发现的主要极性脂分子种类为（每克生物质干重中的毫克总脂肪酸）20:5-16:4-MG，41-2；20:5-16:1-DG，21.0；16:1-16:1-SQ，18-2；和20:5-16:1-MG，18-0。[结构表示甘油分子sn-1位置的脂肪酸 - sn-2位置的脂肪酸 - sn-3位置的碳水化合物成分。
20:5，EPA；16:4，十六碳四烯酸；16:1，棕榈油酸；MG，单半乳糖；DG，双半乳糖；SQ，磺基喹喔啉糖。]
