Oklahoma Blood Institute, Oklahoma City, Oklahoma, USA.
Transfusion. 2010 Apr;50(4):926-31. doi: 10.1111/j.1537-2995.2009.02517.x. Epub 2009 Dec 29.
The Mirasol pathogen reduction technology (PRT) system for plasma is based on a riboflavin (vitamin B(2)) and ultraviolet (UV) light treatment process resulting in pathogen inactivation due to irreversible photo-oxidative damage of nucleic acids. The purpose of this study was to evaluate the in vitro protein quality of apheresis-derived plasma treated with riboflavin and UV light in comparison with untreated fresh-frozen plasma (FFP).
Twenty apheresis plasma samples (270 + or - 10 mL) were combined with 35 + or - 5 mL of riboflavin solution (500 microM), yielding a mean 60 microM final riboflavin concentration, and then exposed to UV light (6.24 J/mL). Riboflavin and UV light-treated plasma was then flash frozen, within 8 hours of collection, generating treated FFP. Treated FFP was thawed and analyzed using standard coagulation assays, and the percent retention of protein activity was reported, relative to untreated, paired controls.
Plasma proteins demonstrated different sensitivities to riboflavin and UV treatment. The amount of total protein remained unchanged. After treatment, fibrinogen (antigen) showed 99% retention; Factor (F)XII, FXIII, ADAMTS-13, and von Willebrand factor (ristocetin cofactor) 96% to 100%. Fibrinogen retained 77% activity, FII 80%, FVIIIc 75%, and FV 73% after treatment. Antithrombin, protein S, plasminogen, and alpha(2)-antiplasmin retained between 91 and 100% activity.
The results from this study demonstrate that coagulant and anticoagulant proteins in riboflavin and UV light-treated (PRT) apheresis plasma are well preserved.
Mirasol 病原体减少技术（PRT）系统用于处理血浆，该技术基于核黄素（维生素 B(2)）和紫外线（UV）光处理过程，由于核酸的不可逆光氧化损伤而导致病原体失活。本研究的目的是评估与未经处理的新鲜冷冻血浆（FFP）相比，用核黄素和紫外线处理的单采血浆的体外蛋白质质量。
将 20 份单采血浆样本（270±10 mL）与 35±5 mL 核黄素溶液（500 μM）混合，得到平均 60 μM 的最终核黄素浓度，然后暴露于紫外线（6.24 J/mL）下。核黄素和紫外线处理后的血浆随后进行闪冻，在收集后 8 小时内生成处理后的 FFP。处理后的 FFP 解冻后，使用标准凝血测定法进行分析，并报告相对于未处理的配对对照，蛋白质活性的保留百分比。
血浆蛋白对核黄素和紫外线处理表现出不同的敏感性。总蛋白量保持不变。处理后，纤维蛋白原（抗原）保留 99%；因子（F）XII、FXIII、ADAMTS-13 和血管性血友病因子（瑞斯托菌素辅因子）保留 96%至 100%。处理后纤维蛋白原保留 77%的活性，FII 80%，FVIIIc 75%，FV 73%。抗凝血酶、蛋白 S、纤溶酶原和 α(2)-抗纤溶酶保留在 91%至 100%之间。
本研究的结果表明，核黄素和紫外线处理（PRT）的单采血浆中的凝血和抗凝蛋白得到了很好的保存。
