Centre for Food-borne and Animal Parasitology, Canadian Food Inspection Agency, Saskatoon, Saskatchewan, Canada S7N 2R3.
J Food Prot. 2010 Jan;73(1):119-24. doi: 10.4315/0362-028x-73.1.119.
Single-strand conformation polymorphism (SSCP) analysis of amplicons produced from a mitochondrial DNA region between the tRNA(Lys) and ATPase8 genes was applied for the detection of animal product within livestock feeds. Identification of prohibited animal (cattle, elk, sheep, deer, and goat) and nonprohibited animal (pig and horse) products from North America was possible based on the differential display of the single-stranded DNA fragments for the different animal species on SSCP gels. This method allowed specific detection and identification of mixed genomic DNA from different animal species. Trace amounts of cattle-derived materials were also detected in pig meat and bone meal and in grain-based feeds fortified with 10, 5, 1, or 0% porcine meat and bone meal. This study demonstrates the applicability of SSCP analyses to successfully identify the origin of animal species derived materials potentially present in animal feeds.
单链构象多态性（SSCP）分析技术被应用于检测牲畜饲料中的动物产品，该技术基于 tRNA（Lys）和 ATPase8 基因之间的线粒体 DNA 区域扩增子的单链构象多态性分析。根据不同动物物种的单链 DNA 片段在 SSCP 凝胶上的差异显示，可鉴定出来自北美的违禁动物（牛、麋鹿、绵羊、鹿和山羊）和非违禁动物（猪和马）产品。该方法可特异性检测和鉴定来自不同动物物种的混合基因组 DNA。在猪骨肉粉和添加 10%、5%、1%或 0%猪骨肉粉的谷物基础饲料中也检测到了痕量的牛源性物质。本研究证明了 SSCP 分析可成功应用于鉴定可能存在于动物饲料中的动物源性物质的来源。
