Technical Center, Guangzhou Customs District People's Republic of China, Guangzhou, China.
Department of Genetics, National Research Institute for Family Planning, Beijing, China.
J Sci Food Agric. 2020 Jan 15;100(1):325-334. doi: 10.1002/jsfa.10042. Epub 2019 Nov 6.
Meat fraud and adulteration incidents occur frequently in almost all regions of the globe, especially with the increase in the world's population. To ensure the authenticity of meat products, we developed a 10-plex xMAP assay to simultaneously detect ten animal materials: bovine, caprine, poultry, swine, donkey, deer, horse, dog, fox and mink.
This method was investigated by analyzing DNA extracts from raw muscle, muscle mixtures, meat products and animal feeds. Our results indicated that the species of interest can be identified, differentiated and detected down to 1 g kg in binary mixtures or 0.01-0.001 ng of genomic DNA from specific species. Testing of 125 commercial samples showed a 97.4% coincidence rate with the method used in routine testing in our lab.
These results indicated that the method established in this study could detect ten animal materials simultaneously within 3 h, which provides a new, useful tool for animal ingredient analysis in meat products and animal feeds. © 2019 Society of Chemical Industry.
肉类欺诈和掺假事件几乎在全球所有地区都频繁发生，尤其是随着世界人口的增加。为了确保肉类产品的真实性，我们开发了一种 10 重 xMAP 检测方法，可同时检测十种动物材料：牛、羊、禽、猪、驴、鹿、马、狗、狐和貂。
通过分析来自生肌肉、肌肉混合物、肉类产品和动物饲料的 DNA 提取物，对该方法进行了研究。我们的结果表明，感兴趣的物种可以被识别、区分和检测，在二元混合物中低至 1g/kg，或从特定物种的 0.01-0.001ng 基因组 DNA 中检测到。对 125 个商业样本的测试表明，与我们实验室常规测试中使用的方法的符合率为 97.4%。
这些结果表明，本研究中建立的方法可以在 3 小时内同时检测十种动物材料，为肉类产品和动物饲料中的动物成分分析提供了一种新的有用工具。 © 2019 化学工业协会。
