National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing, P.R. China.
Hum Exp Toxicol. 2010 Jun;29(6):497-506. doi: 10.1177/0960327109357213. Epub 2010 Jan 5.
To explore the effect of 2,5-hexanedione on permeability of blood-nerve barrier, adult Wistar rats were administered with 400 mg x kg(-1) x d(- 1) 2,5-hexanedione to establish animal model of 2,5-hexnedione neuropathy. Evans blue was injected through left femoral vein of the rats after the model had been established. The distribution of fluorescence in sciatic-tibial nerve was observed and assessed. For the transverse sections of sciatic-tibial nerves, the average fluorescence intensity of proximal section was stronger (p < .01) than those of intermediate and distal sections and the average fluorescence intensity of intermediate section was stronger (p < .01) than that of distal section in the intoxicated group. In the control, the weak fluorescence was shown, and average fluorescence intensity of distal section was stronger (p < .05) than that of proximal section. The average fluorescence intensity of proximal, intermediate and distal sections in the intoxicated group was stronger (p < .01) than those of the corresponding sections in the control. For the longitudinal sections of sciatic-tibial nerves, fluorescence was observed in both proximal and distal sections in the intoxicated group. The fluorescence intensity of distal section in the control was weak and almost no fluorescence was shown in the proximal section. The permeability of blood-nerve barrier could be increased by 2,5-hexanedione.
为了探讨 2,5-己二酮对血神经屏障通透性的影响，将成年 Wistar 大鼠给予 400mg·kg-1·d-1的 2,5-己二酮，建立 2,5-己二酮神经病变动物模型。造模成功后，通过左侧股静脉向大鼠体内注射 Evans 蓝。观察并评估坐骨-胫神经中荧光的分布。对于坐骨-胫神经的横切片，中毒组的近段平均荧光强度较强（p<.01），中段和远段平均荧光强度较弱（p<.01），中段平均荧光强度较强（p<.01），远段平均荧光强度较弱。在对照组中，显示出较弱的荧光，远段的平均荧光强度较强（p<.05），近段平均荧光强度较弱。中毒组近段、中段和远段的平均荧光强度均强于对照组相应节段（p<.01）。对于坐骨-胫神经的纵切片，中毒组的近段和远段均观察到荧光。对照组近段荧光很弱，几乎没有荧光显示。2,5-己二酮可增加血神经屏障的通透性。
