Liu Qingjun, Zhao Lei, Duan Huawei, Dai Yufei, Niu Yong, Chen Hong, Liu Qing, Bin Ping, Zheng Yuxin
National Institute for Occupational Health and Poison Control, Chinese Center for Disease Control and Prevention, Beijing 100050, China.
Wei Sheng Yan Jiu. 2010 May;39(3):275-8.
To explore the effects of 2,5-hexandione, the metabolite of n-hexane, on the expression of myelin protein zero (P0) in sciatic nerve and on the positive rate of P0 antibody in serum of rats at different time points.
Seventy five Wistar rats were divided into five groups and were administrated with 400 mg/kg 2,5-hexanedione per day for 0, 1, 2, 3 and 4 weeks respectively. The P0 expression at different time points was determined with immunohischemistry and the P0 antibody in serum were detected with enzyme-linked immunosorbent assay.
With the administration of 2,5-hexandione, the rats gradually showed the signs of peripheral neuropathy. P0 distribution in transverse section of sciatic nerve was different, and the intensity in myelin sheath was higher than that in axon. The expression of P0 in sciatic nerve of rats with 2,5-hexanedione administration for 0 week seemed higher than those of the other time points, and the expression of the P0 showed a decreasing tendency with the time of 2,5-hexanedione administration. The positive rate of P0 antibody in serum of rats administrated with 400 mg/kg 2,5-hexanedione for 0, 1, 2, 3 and 4 weeks were 33.3%, 26.7%, 46.7%, 46.7% and 84.6% respectively. The positive rate of Po antibodies in serum of rats showed an increasing tendency with the time of 2,5-hexanedione administration (chi2 = 11.007, P < 0.05).
The P0 in sciatic nerve of rats could be destroyed by 2,5-hexanedione and P0 expression level decreased with the time of 2,5-hexanedione administration. The positive rate of P0 antibody in serum increased with the time of 2,5-hexanedione administration in rats.
探讨正己烷代谢产物2,5 - 己二酮在不同时间点对大鼠坐骨神经髓鞘蛋白零（P0）表达及血清中P0抗体阳性率的影响。
将75只Wistar大鼠分为5组，分别给予400mg/kg 2,5 - 己二酮，连续给药0、1、2、3和4周。采用免疫组织化学法检测不同时间点的P0表达，酶联免疫吸附测定法检测血清中的P0抗体。
随着2,5 - 己二酮的给药，大鼠逐渐出现周围神经病变的体征。坐骨神经横切面中P0分布不同，髓鞘中的强度高于轴突。给予2,5 - 己二酮0周的大鼠坐骨神经中P0表达似乎高于其他时间点，且P0表达随2,5 - 己二酮给药时间呈下降趋势。给予400mg/kg 2,5 - 己二酮0、1、2、3和4周的大鼠血清中P0抗体阳性率分别为33.3%、26.7%、46.7%、46.7%和84.6%。大鼠血清中P0抗体阳性率随2,5 - 己二酮给药时间呈上升趋势（χ2 = 11.007，P < 0.05）。
2,5 - 己二酮可破坏大鼠坐骨神经中的P0，且P0表达水平随2,5 - 己二酮给药时间下降。大鼠血清中P0抗体阳性率随2,5 - 己二酮给药时间增加。
