GAD67-GFP基因敲入小鼠具有正常的睡眠-觉醒模式和睡眠稳态。
GAD67-GFP knock-in mice have normal sleep-wake patterns and sleep homeostasis.
作者信息
Chen Lichao, McKenna James T, Leonard Michael Z, Yanagawa Yuchio, McCarley Robert W, Brown Ritchie E
机构信息
Laboratory of Neuroscience, VA Boston Healthcare System and Department of Psychiatry, Harvard Medical School, Brockton, Massachusetts, USA.
出版信息
Neuroreport. 2010 Feb 17;21(3):216-20. doi: 10.1097/WNR.0b013e32833655c4.
Abstract
Gamma-aminobutyric acid (GABA) ergic neurons are important for controlling sleep and wakefulness but are difficult to identify, limiting their study. Knock-in mice with GABAergic neurons labeled by expression of green fluorescent protein (GFP) under control of the glutamate decarboxylase 67 (GAD67) promoter are now extensively used in neuroscience. However, it is unknown whether these mice have a normal sleep phenotype. Compared with wild-type control mice, GAD67-GFP knock-in mice had the same amount of non-rapid eye movement (NREM) sleep and rapid-eye movement (REM) sleep, a similar diurnal distribution of sleep, no NREM or REM sleep differences in electroencephalogram power, and normal sleep rebound following 6-h sleep deprivation. Our results suggest GAD67-GFP knock-in mice are an excellent tool for study of GABAergic neurons involved in sleep-wake regulation.
摘要
γ-氨基丁酸（GABA）能神经元对控制睡眠和觉醒很重要，但难以识别，这限制了对它们的研究。在谷氨酸脱羧酶67（GAD67）启动子控制下通过绿色荧光蛋白（GFP）表达标记GABA能神经元的敲入小鼠，目前在神经科学中被广泛使用。然而，尚不清楚这些小鼠是否具有正常的睡眠表型。与野生型对照小鼠相比，GAD67-GFP敲入小鼠的非快速眼动（NREM）睡眠和快速眼动（REM）睡眠量相同，睡眠的昼夜分布相似，脑电图功率在NREM或REM睡眠中无差异，并且在6小时睡眠剥夺后有正常的睡眠反弹。我们的结果表明，GAD67-GFP敲入小鼠是研究参与睡眠-觉醒调节的GABA能神经元的优秀工具。
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