Wang Jiale, Ding Chong, Fu Rui, Zhang Ze, Zhao Junqiao, Zhu Haijun
School of Health Sciences & Biomedical Engineering, Hebei University of Technology, Tianjin 300130, P. R. China.
Hebei Key Laboratory of Bioelectromagnetics and Neural Engineering, Hebei University of Technology, Tianjin 300130, P. R. China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2025 Feb 25;42(1):73-81. doi: 10.7507/1001-5515.202405025.
Repeated transcranial magnetic stimulation (rTMS) is one of the commonly used brain stimulation techniques. In order to investigate the effects of rTMS on the excitability of different types of neurons, this study is conducted to investigate the effects of rTMS on the cognitive function of mice and the excitability of hippocampal glutaminergic neurons and gamma-aminobutyric neurons from the perspective of electrophysiology. In this study, mice were randomly divided into glutaminergic control group, glutaminergic magnetic stimulation group, gamma-aminobutyric acid energy control group, and gamma-aminobutyric acid magnetic stimulation group. The four groups of mice were injected with adeno-associated virus to label two types of neurons and were implanted optical fiber. The stimulation groups received 14 days of stimulation and the control groups received 14 days of pseudo-stimulation. The fluorescence intensity of calcium ions in mice was recorded by optical fiber system. Behavioral experiments were conducted to explore the changes of cognitive function in mice. The patch-clamp system was used to detect the changes of neuronal action potential characteristics. The results showed that rTMS significantly improved the cognitive function of mice, increased the amplitude of calcium fluorescence of glutamergic neurons and gamma-aminobutyric neurons in the hippocampus, and enhanced the action potential related indexes of glutamergic neurons and gamma-aminobutyric neurons. The results suggest that rTMS can improve the cognitive ability of mice by enhancing the excitability of hippocampal glutaminergic neurons and gamma-aminobutyric neurons.
重复经颅磁刺激（rTMS）是常用的脑刺激技术之一。为了研究rTMS对不同类型神经元兴奋性的影响，本研究从电生理学角度探讨rTMS对小鼠认知功能以及海马谷氨酰胺能神经元和γ-氨基丁酸能神经元兴奋性的影响。在本研究中，小鼠被随机分为谷氨酰胺能对照组、谷氨酰胺能磁刺激组、γ-氨基丁酸能对照组和γ-氨基丁酸能磁刺激组。四组小鼠均注射腺相关病毒以标记两种类型的神经元，并植入光纤。刺激组接受14天的刺激，对照组接受14天的假刺激。通过光纤系统记录小鼠体内钙离子的荧光强度。进行行为实验以探索小鼠认知功能的变化。使用膜片钳系统检测神经元动作电位特征的变化。结果表明，rTMS显著改善了小鼠的认知功能，增加了海马中谷氨酰胺能神经元和γ-氨基丁酸能神经元的钙荧光幅度，并增强了谷氨酰胺能神经元和γ-氨基丁酸能神经元的动作电位相关指标。结果提示，rTMS可通过增强海马谷氨酰胺能神经元和γ-氨基丁酸能神经元的兴奋性来提高小鼠的认知能力。
