Department of Orthopedics, Shengjing Hospital, China Medical University, 36 San Hao Street, Shenyang, Liaoning 110004, China.
Ann Biomed Eng. 2010 Apr;38(4):1647-54. doi: 10.1007/s10439-009-9892-x. Epub 2010 Jan 6.
Articular cartilage defects are common, causing significant morbidities. Tissue engineering using pluripotent stem cells is a new promising modality for cartilage repair. In the current study, we investigated the chondrogenesis of rabbit adipose-derived stem cells (ADSCs). We isolated rabbit ADSCs and transfected these cells with constructs encoding human insulin growth like factor 1 (IGF-1) and bone morphogenic protein 2 (BMP-2). We examined the growth and morphology of these transfected cells and their production of type II collagen and MMP-3. We found that IGF-1 and BMP-2 drove the chondrogenesis of ADSCs, which showed mature chondrocyte-like cells and formed cartilage nodules. These cells also produced type II collagen with a reduced production of MMP-3. Our findings suggested that human ADSCs could differentiate into chondrocyte-like cells driven by IGF-1 and BMP-2 and held promises as an abundant and ready source of stem cells for cartilage repair and regeneration.
关节软骨缺损很常见，会导致严重的病况。利用多能干细胞进行组织工程是一种修复软骨的新的有前途的方法。在本研究中，我们研究了兔脂肪来源干细胞（ADSCs）的软骨生成。我们分离了兔 ADSCs，并将这些细胞转染了编码人胰岛素样生长因子 1（IGF-1）和骨形态发生蛋白 2（BMP-2）的构建体。我们检查了这些转染细胞的生长和形态，以及它们产生的 II 型胶原和 MMP-3。我们发现 IGF-1 和 BMP-2 驱动 ADSCs 的软骨生成，这些细胞表现出成熟的软骨细胞样细胞，并形成软骨结节。这些细胞还产生了 II 型胶原，同时减少了 MMP-3 的产生。我们的研究结果表明，人 ADSCs 可以在 IGF-1 和 BMP-2 的驱动下分化为软骨细胞样细胞，有望成为软骨修复和再生的丰富且现成的干细胞来源。
