用于评估发生过敏反应的血液透析患者对环氧乙烷 - 人血清白蛋白反应性的皮肤试验与酶联免疫吸附测定试验的比较。

A comparison of cutaneous testing and ELISA testing for assessing reactivity to ethylene oxide-human serum albumin in hemodialysis patients with anaphylactic reactions.

作者信息

Grammer L C, Roberts M, Wiggins C A, Fitzsimons R R, Ivanovich P T, Roxe D M, Patterson R

机构信息

Department of Medicine, Northwestern University Medical School, Chicago, Ill 60611.

出版信息

J Allergy Clin Immunol. 1991 Mar;87(3):674-6. doi: 10.1016/0091-6749(91)90387-4.

DOI:10.1016/0091-6749(91)90387-4

PMID:2005319

Abstract

In five patients who have experienced anaphylaxis and in 29 patients who have not had such episodes during hemodialysis, we have performed two immunologic studies: cutaneous testing with ethylene oxide-human serum albumin (ETO-HSA) and ELISA for IgE against ETO-HSA. Four of five patients with reactions had positive cutaneous tests, whereas only one nonreactor had a positive skin test (p less than 0.0002). The same four of five patients with reactions also had positive ELISA results, whereas three nonreactors has positive ELISA results (p less than 0.003). In this group of patients, the positive predictive value of cutaneous testing (80%) is somewhat higher than that of ELISA testing (57%). However, the sensitivity, specificity, and negative predictive values are similar. We conclude that cutaneous testing with ETO-HSA probably offers a small advantage over IgE against ETO-HSA as determined by ELISA.

摘要

在5例有过敏反应的患者以及29例血液透析期间未发生此类反应的患者中，我们进行了两项免疫学研究：用环氧乙烷 - 人血清白蛋白（ETO - HSA）进行皮肤试验以及针对ETO - HSA的IgE酶联免疫吸附测定（ELISA）。5例有反应的患者中有4例皮肤试验呈阳性，而在无反应的患者中只有1例皮肤试验呈阳性（p < 0.0002）。5例有反应的患者中的同样4例ELISA结果也呈阳性，而3例无反应者ELISA结果呈阳性（p < 0.003）。在这组患者中，皮肤试验的阳性预测值（80%）略高于ELISA检测（57%）。然而，敏感性、特异性和阴性预测值相似。我们得出结论，用ETO - HSA进行皮肤试验可能比ELISA测定针对ETO - HSA的IgE具有些许优势。