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儿子对于核斑点的组织和细胞周期的进展是必不可少的。

Son is essential for nuclear speckle organization and cell cycle progression.

机构信息

Biomedical Sciences Ph.D. Program, Department of Biological Sciences, Wright State University, Dayton, OH 45435, USA.

出版信息

Mol Biol Cell. 2010 Feb 15;21(4):650-63. doi: 10.1091/mbc.e09-02-0126. Epub 2010 Jan 6.

DOI:10.1091/mbc.e09-02-0126
PMID:20053686
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2820428/
Abstract

Subnuclear organization and spatiotemporal regulation of pre-mRNA processing factors is essential for the production of mature protein-coding mRNAs. We have discovered that a large protein called Son has a novel role in maintaining proper nuclear organization of pre-mRNA processing factors in nuclear speckles. The primary sequence of Son contains a concentrated region of multiple unique tandem repeat motifs that may support a role for Son as a scaffolding protein for RNA processing factors in nuclear speckles. We used RNA interference (RNAi) approaches and high-resolution microscopy techniques to study the functions of Son in the context of intact cells. Although Son precisely colocalizes with pre-mRNA splicing factors in nuclear speckles, its depletion by RNAi leads to cell cycle arrest in metaphase and causes dramatic disorganization of small nuclear ribonuclear protein and serine-arginine rich protein splicing factors during interphase. Here, we propose that Son is essential for appropriate subnuclear organization of pre-mRNA splicing factors and for promoting normal cell cycle progression.

摘要

前体 mRNA 处理因子的亚核组织和时空调控对于成熟蛋白编码 mRNA 的产生至关重要。我们发现一种名为 Son 的大型蛋白在维持核斑点中前体 mRNA 处理因子的核组织方面具有新的作用。Son 的一级序列包含多个独特串联重复基序的浓缩区域，这可能支持 Son 作为核斑点中 RNA 处理因子支架蛋白的作用。我们使用 RNA 干扰 (RNAi) 方法和高分辨率显微镜技术，在完整细胞的背景下研究 Son 的功能。尽管 Son 与核斑点中的前体 mRNA 剪接因子精确共定位，但 RNAi 使其耗竭会导致细胞周期在中期停滞，并导致小核核糖核蛋白和丝氨酸-精氨酸丰富蛋白剪接因子在间期出现明显的紊乱。在这里，我们提出 Son 对于前体 mRNA 剪接因子的适当亚核组织和促进正常细胞周期进程是必不可少的。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/299b03762254/zmk0041093590012.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/4d45c7b30710/zmk0041093590006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/1a3963ee6eda/zmk0041093590007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/83e601f93c36/zmk0041093590008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/6546421e181a/zmk0041093590009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/a48ae9c5605b/zmk0041093590010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/9d8a8cbe7b5a/zmk0041093590011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/299b03762254/zmk0041093590012.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/03c5c1f00ec6/zmk0041093590001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/37510fbb0d2d/zmk0041093590002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/480116405717/zmk0041093590003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/ebe1d3d4766a/zmk0041093590004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/3077ff497206/zmk0041093590005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/4d45c7b30710/zmk0041093590006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/1a3963ee6eda/zmk0041093590007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/83e601f93c36/zmk0041093590008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/6546421e181a/zmk0041093590009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/a48ae9c5605b/zmk0041093590010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/9d8a8cbe7b5a/zmk0041093590011.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e2a5/2820428/299b03762254/zmk0041093590012.jpg

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