Knight E, Cordova B
Central Research and Development Department, E. I. du Pont de Nemours and Company, Wilmington, DE 19880-0328.
J Immunol. 1991 Apr 1;146(7):2280-4.
The enhancement or inhibition of synthesis of specific proteins by IFN is believed to cause subsequent IFN-induced biological responses. The roles of most of these proteins in the biological responses induced by the IFNs, for example, inhibition of virus replication and inhibition of cell growth, remain largely unknown. Our recent research has focused on the induction and synthesis of an IFN-induced 15-kDa protein. In this report we show that human lymphocytes and monocytes, after treatment with IFN-beta, release into the medium an IFN-induced 15-kDa protein. At 24 h after induction of the 15-kDa protein in lymphocytes or monocytes, more than 50% of the total 15-kDa protein is in the medium. The human monocytic cell line THP-1 also releases 15-kDa protein into the medium after its induction by IFN-beta. An intracellular half-life of 12 h has been calculated for the 15-kDa protein in monocytes and THP-1 cells. The exocellular release of the 15-kDa protein by lymphocytes and monocytes suggests that 1) it may have an intercellular signaling role and 2) it may be an in vivo mediator of some of the biological responses induced by IFN.
干扰素对特定蛋白质合成的增强或抑制作用被认为会引发随后由干扰素诱导的生物学反应。这些蛋白质中的大多数在干扰素诱导的生物学反应中的作用，例如抑制病毒复制和抑制细胞生长，在很大程度上仍然未知。我们最近的研究集中在一种干扰素诱导的15 kDa蛋白质的诱导和合成上。在本报告中，我们表明，用β干扰素处理后的人淋巴细胞和单核细胞会向培养基中释放一种干扰素诱导的15 kDa蛋白质。在淋巴细胞或单核细胞中诱导出15 kDa蛋白质后24小时，超过50%的总15 kDa蛋白质存在于培养基中。人单核细胞系THP-1在被β干扰素诱导后也会向培养基中释放15 kDa蛋白质。已计算出单核细胞和THP-1细胞中15 kDa蛋白质的细胞内半衰期为12小时。淋巴细胞和单核细胞对15 kDa蛋白质的细胞外释放表明：1）它可能具有细胞间信号传导作用；2）它可能是干扰素诱导的一些生物学反应的体内介质。
