Department of Dental Pharmacology, School of Dentistry, and Institute of Oral Bioscience, Brain Korea 21 Project, Chonbuk National University, Jeon-Ju 561-756, Korea.
Korean J Physiol Pharmacol. 2009 Dec;13(6):417-24. doi: 10.4196/kjpp.2009.13.6.417. Epub 2009 Dec 31.
Osteoclasts, derived from multipotent myeloid progenitor cells, play homeostatic roles in skeletal modeling and remodeling, but may also destroy bone in pathological conditions such as osteoporosis and rheumatoid arthritis. Osteoclast development depends critically on a differentiation factor, the receptor activator of NF-kappaB ligand (RANKL). In this study, we found that the hexane soluble fraction of the common fig Ficus carica (HF6-FC) is a potent inhibitor of osteoclastogenesis in RANKL-stimulated RAW264.7 cells and in bone marrow-derived macrophages (BMMs). HF6-FC exerts its inhibitory effects by suppression of p38 and NF-kappaB but activation of ERK. In addition, HF6-FC significantly decreased the expression of NFATc1 and c-Fos, the master regulator of osteoclast differentiation. The data indicate that components of HF6-FC may have therapeutic effects on bone-destructive processes such as osteoporosis, rheumatoid arthritis, and periodontal bone resorption.
破骨细胞来源于多能髓样前体细胞，在骨骼建模和重塑中发挥着动态平衡的作用，但在骨质疏松症和类风湿关节炎等病理条件下也可能破坏骨骼。破骨细胞的发育取决于分化因子，核因子 κB 配体受体激活剂（RANKL）。在这项研究中，我们发现榕属植物无花果的正己烷可溶部分（HF6-FC）是一种有效的 RANKL 刺激 RAW264.7 细胞和骨髓来源的巨噬细胞（BMM）中破骨细胞发生的抑制剂。HF6-FC 通过抑制 p38 和 NF-κB 但激活 ERK 来发挥其抑制作用。此外，HF6-FC 显著降低了破骨细胞分化的主调控因子 NFATc1 和 c-Fos 的表达。数据表明，HF6-FC 的成分可能对骨质疏松症、类风湿关节炎和牙周骨吸收等破坏性骨过程具有治疗作用。
