Eteraf-Oskouei Tahereh, Allahyari Saeideh, Akbarzadeh-Atashkhosrow Arezu, Delazar Abbas, Pashaii Mahdiyeh, Gan Siew Hua, Najafi Moslem
Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz 5166414766, Iran ; Department of Pharmacology and Toxicology, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 5166414766, Iran.
Student Research Committee, Faculty of Pharmacy, Tabriz University of Medical Sciences, Tabriz 5166414766, Iran.
Evid Based Complement Alternat Med. 2015;2015:760405. doi: 10.1155/2015/760405. Epub 2015 Apr 21.
The antiangiogenesis effect of Ficus carica leaves extract in an air pouch model of inflammation was investigated in rat. Inflammation was induced by injection of carrageenan into pouches. After antioxidant capacity and total phenolic content (TPC) investigations, the extract was administered at 5, 25, and 50 mg/pouch, and then the volume of exudates, the cell number, TNFα, PGE2, and VEGF levels were measured. Angiogenesis of granulation tissues was determined by measuring hemoglobin content. Based on the DPPH assay, the extract had significant antioxidant activity with TPC of 11.70 mg GAE/100 g dry sample. In addition, leukocyte accumulation and volume of exudate were significantly inhibited by the extract. Moreover, it significantly decreased the production of TNFα, PGE2, and VEGF, while angiogenesis was significantly inhibited by all administered doses. Interestingly, attenuation of angiogenesis and inflammatory parameters (except leukocyte accumulation) by the extract was similar to that shown by diclofenac. The extract has anti-inflammatory effects and ameliorated cell influx and exudation to the site of the inflammatory response which may be related to the local inhibition of TNFα, PGE2, and VEGF levels as similarly shown by diclofenac. The antiangiogenesis and anti-VEGF effects of Ficus carica may be correlated with its significant antioxidant potentials.
在大鼠气袋炎症模型中研究了无花果叶提取物的抗血管生成作用。通过向气袋中注射角叉菜胶诱导炎症。在对抗氧化能力和总酚含量（TPC）进行研究后，分别以5、25和50mg/气袋的剂量给予提取物，然后测量渗出液体积、细胞数量、TNFα、PGE2和VEGF水平。通过测量血红蛋白含量来确定肉芽组织的血管生成情况。基于DPPH法，提取物具有显著的抗氧化活性，TPC为11.70mg GAE/100g干样品。此外，提取物显著抑制白细胞积聚和渗出液体积。而且，它显著降低TNFα、PGE2和VEGF的产生，同时所有给药剂量均显著抑制血管生成。有趣的是，提取物对血管生成和炎症参数（白细胞积聚除外）的抑制作用与双氯芬酸相似。该提取物具有抗炎作用，可改善炎症反应部位的细胞流入和渗出，这可能与局部抑制TNFα、PGE2和VEGF水平有关，双氯芬酸也有类似表现。无花果的抗血管生成和抗VEGF作用可能与其显著的抗氧化潜力相关。
