Ref-1 的过表达通过上调过氧化氢酶抑制铅诱导的内皮细胞死亡。
Overexpression of Ref-1 Inhibits Lead-induced Endothelial Cell Death via the Upregulation of Catalase.
机构信息
Department of Physiology, School of Medicine, Chungnam National University, Daejeon 301-131, Korea.
出版信息
Korean J Physiol Pharmacol. 2009 Dec;13(6):431-6. doi: 10.4196/kjpp.2009.13.6.431. Epub 2009 Dec 31.
Abstract
The role of apurinic/apyrimidinic endonuclease1/redox factor-1 (Ref-1) on the lead (Pb)-induced cellular response was investigated in the cultured endothelial cells. Pb caused progressive cellular death in endothelial cells, which occurred in a concentration- and time-dependent manner. However, Ref-1 overexpression with AdRef-1 significantly inhibited Pb-induced cell death in the endothelial cells. Also the overexpression of Ref-1 significantly suppressed Pb-induced superoxide and hydrogen peroxide elevation in the endothelial cells. Pb exposure induced the downregulation of catalase, it was inhibited by the Ref-1 overexpression in the endothelial cells. Taken together, our data suggests that the overexpression of Ref-1 inhibited Pb-induced cell death via the upregulation of catalase in the cultured endothelial cells.
摘要
研究了脱嘌呤/脱嘧啶核酸内切酶 1/氧化还原因子-1（Ref-1）在培养的内皮细胞中铅（Pb）诱导的细胞反应中的作用。Pb 导致内皮细胞进行性细胞死亡，其发生呈浓度和时间依赖性。然而，AdRef-1 过表达显著抑制了内皮细胞中 Pb 诱导的细胞死亡。Ref-1 的过表达也显著抑制了 Pb 诱导的内皮细胞中超氧阴离子和过氧化氢的升高。Pb 暴露诱导过氧化氢酶下调，Ref-1 的过表达抑制了内皮细胞中的过氧化氢酶下调。总之，我们的数据表明，过表达 Ref-1 通过在上皮细胞中上调过氧化氢酶来抑制 Pb 诱导的细胞死亡。
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