Oncology Research Unit, Hospital Oncologia, Health Research Coordination, IMSS, Mexico City, Mexico.
J Cancer Res Clin Oncol. 2010 Jun;136(6):931-8. doi: 10.1007/s00432-009-0735-9. Epub 2010 Jan 7.
To investigate the genomic alterations in larynx carcinomas (LaCa) tissues and its prognostics values in predicting survival.
To analyse the aberrations in the genome of LaCa patients, we used array comparative genomic hybridization in 19 human laryngeal tumour samples. DNA samples were also subjected to detect human papillomavirus (HPV) sequences by polymerase chain reaction (PCR). Copy number gain was confirmed by real-time PCR. The cellular retinol-binding protein 1 (CRBP-1) gene expression was also confirmed by immunohistochemistry assay on LaCa tissues. To identify prognostic feature, CRBP-1 gene gain was correlated to patient survival.
The most common gains were detected for CRBP-1 and EGFR genes, while DNA lost in RAF-1 gene. Immunohistochemistry assay was revealed strong expression of CRBP1 protein in those cases with CRBP-1 gene gain. The CRBP-1 gene gain and its expression correlated significantly with survival (P = 0.003). Cox regression analysis indicated that CRBP-1 expression level was a factor of survival (P = 0.008). HPV sequences were detected in 42% of the samples, and did not show any relationship with specific gene alterations.
Our data shows that CRBP-1 gene gain can be determined by immunohistochemistry on routinely processed tissue specimens, and could support as a potential novel marker for long-term survival in laryngeal squamous cell carcinoma.
研究喉癌（LaCa）组织中的基因组改变及其在预测生存中的预后价值。
为了分析 LaCa 患者基因组的异常，我们使用阵列比较基因组杂交技术对 19 个人类喉肿瘤样本进行了分析。还通过聚合酶链反应（PCR）检测了 DNA 样本中人乳头瘤病毒（HPV）序列。通过实时 PCR 证实了拷贝数的增加。还通过免疫组织化学法在 LaCa 组织上证实了细胞视黄醇结合蛋白 1（CRBP-1）基因的表达。为了确定预后特征，将 CRBP-1 基因增益与患者生存相关联。
最常见的增益检测到了 CRBP-1 和 EGFR 基因，而 RAF-1 基因则丢失了 DNA。免疫组织化学检测显示，在 CRBP-1 基因增益的情况下，CRBP1 蛋白的表达很强。CRBP-1 基因增益及其表达与生存显著相关（P = 0.003）。Cox 回归分析表明，CRBP-1 表达水平是生存的一个因素（P = 0.008）。在 42%的样本中检测到了 HPV 序列，但与特定基因改变没有任何关系。
我们的数据表明，CRBP-1 基因增益可以通过常规处理的组织标本的免疫组织化学来确定，并且可以作为长期生存的潜在新型标志物支持喉鳞状细胞癌。
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