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伊朗微生物实验室对细菌性脑膜炎病原体鉴定的评估。一项外部质量评估计划(EQAS)的调查结果。

Evaluation of Iranian microbiology laboratories for identification of etiologic agents of bacterial meningitidis. Survey results of an external quality assessment scheme (EQAS) programme.

作者信息

Marandi Farinaz Rashed, Rahbar Mohammad, Sabourian Roghieh, Saremi Mahnaz

机构信息

Department of Microbiology, Iranian Health Reference Laboratory, Ministry of Health and Medical Education, Tehran, Iran.

出版信息

J Pak Med Assoc. 2010 Jan;60(1):48-51.

PMID:20055281
Abstract

OBJECTIVE

To determine the ability of Iranian microbiology laboratories for identification and susceptibility testing of Streptococcus pneumoniae and Haemophilus influenzae as causative agents of bacterial meningitides.

METHODS

Two strains of bacteria including Haemophilus influenzae and Streptococcus pneumoniae as a common causative agents of meningitides were chosen and coded as strain number 1 and number 2. The strains were distributed among 679 microbiology laboratories. All laboratories were requested for identification of each unknown microorganism and susceptibility testing of S. pneumoniae against five commonly used antibiotics.

RESULTS

Of 679 microbiology laboratories 310 (46%) laboratories participated in the survey and among these, 258 laboratories completely identified S. pneumoniae. About 85% laboratories produced correct susceptibility testing against oxacillin, erythromycin, tetracycline, and vancomycin. Of 310 received responses only 50 laboratories identified H. influenza correctly. The majority of the laboratories did not have the capacity to identification H. influenza.

CONCLUSION

Microbiology laboratories in our country are qualified for identification and susceptibility testing of S. pneumoniae. However, majority of laboratories are not qualified for identification of H. influenzae.

摘要

目的

确定伊朗微生物实验室对作为细菌性脑膜炎病原体的肺炎链球菌和流感嗜血杆菌进行鉴定及药敏试验的能力。

方法

选择两种作为脑膜炎常见病原体的细菌,即流感嗜血杆菌和肺炎链球菌,分别编码为菌株1号和2号。将这些菌株分发给679家微生物实验室。要求所有实验室对每种未知微生物进行鉴定,并对肺炎链球菌进行针对五种常用抗生素的药敏试验。

结果

679家微生物实验室中,310家(46%)参与了调查,其中258家实验室完全鉴定出了肺炎链球菌。约85%的实验室对苯唑西林、红霉素、四环素和万古霉素进行了正确的药敏试验。在收到回复的310家实验室中,只有50家正确鉴定出了流感嗜血杆菌。大多数实验室没有鉴定流感嗜血杆菌的能力。

结论

我国微生物实验室有能力对肺炎链球菌进行鉴定和药敏试验。然而,大多数实验室没有能力鉴定流感嗜血杆菌。

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