Department of Dermatology, Shiga University of Medical Science, Shiga, Japan.
Clin Exp Dermatol. 2009 Dec;34(8):e965-7. doi: 10.1111/j.1365-2230.2009.03708.x.
Human papillomavirus (HPV) is an important aetiological agent in cervical carcinomas and in malignant skin tumours. Integration of the HPV DNA into host genome is one of the most important risk factors for malignant transformation. We report a patient with multiple black plaques and an erythematous nodule on her vulva. On histological examination, multifocal vulvar Bowen's disease (BD) and invasive squamous cell carcinoma (SCC) were found. An amplification of papillomavirus oncogene transcripts (APOT) assay showed that two locations of BD had only episome-derived HPV16 transcripts, but the other two sites of BD and the nodule of invasive SCC had HPV16 transcripts derived from integration. Sequencing analysis revealed that the invasive SCC had its integration site at 8q24, the Myc locus. Our results suggest that the APOT assay in multiple sites of the same patient may be a valuable tool for evaluation of the clinical degree of malignancy for vulvar BD.
人乳头瘤病毒（HPV）是宫颈癌和恶性皮肤肿瘤的重要病因。HPV DNA 整合到宿主基因组是恶性转化的最重要危险因素之一。我们报告了一位患者，其外阴有多个黑色斑块和一个红斑性结节。组织学检查发现多灶性外阴鲍恩病（BD）和浸润性鳞状细胞癌（SCC）。乳头状瘤病毒癌基因转录物扩增（APOT）检测显示，BD 的两个部位仅有来自于游离体的 HPV16 转录物，但 BD 的另外两个部位和浸润性 SCC 的结节有来自整合的 HPV16 转录物。测序分析显示，浸润性 SCC 的整合部位在 8q24，即 Myc 基因座。我们的结果表明，同一患者多个部位的 APOT 检测可能是评估外阴 BD 临床恶性程度的有用工具。
