Molecular Biology and Viral Oncology and AIDS Reference Centre, National Cancer Institute Fondazione Pascale, Cappella Cangiani, 80131, Naples, Italy.
Pathol Oncol Res. 2012 Oct;18(4):803-8. doi: 10.1007/s12253-012-9507-y. Epub 2012 Mar 27.
Oncogenic HPVs have been found frequently integrated into human genome of invasive cancers and chromosomal localization has been extensively investigated in cervical carcinoma. Few studies have analyzed the HPV integration loci in other genital cancers. We have characterized the integration sites of HPV16 in invasive penile carcinoma by means of Alu-HPV-based PCR. Nucleotide sequence analysis of viral-human DNA junctions showed that HPV integration occurred in one case within the chromosome 8q21.3 region, in which the FAM92A1 gene is mapped, and in the second case inside the chromosome 16p13.3, within the intronic region of TRAP1 gene. These results confirm previous observations, summarized in a systematic review of the literature, on the HPV integration events in gene loci relevant to cancer pathogenesis.
致癌型 HPV 经常被发现整合到侵袭性癌症的人类基因组中，在宫颈癌中已广泛研究了染色体定位。很少有研究分析过其他生殖系统癌症中的 HPV 整合部位。我们通过基于 Alu-HPV 的 PCR 方法对侵袭性阴茎癌中的 HPV16 整合部位进行了特征分析。病毒-人 DNA 连接处的核苷酸序列分析表明，HPV 整合发生在一个病例中，位于染色体 8q21.3 区域内，映射了 FAM92A1 基因，在第二个病例中位于染色体 16p13.3 内，TRAP1 基因的内含子区域内。这些结果证实了文献系统综述中总结的先前关于 HPV 在与癌症发病机制相关基因座中整合事件的观察结果。
