Wu Yin-Liang, Li Cun, Xia Xi, Liu Yong-Jun, Shen Jian-Zhong
College of Veterinary Medicine, China Agricultural University, Beijing 100094, People's Republic of China.
J Chromatogr Sci. 2010 Jan;48(1):63-7. doi: 10.1093/chromsci/48.1.63.
A simple and sensitive high-performance liquid chromatography (HPLC) analytical method for the simultaneous determination of six Sudan dyes (Sudan Red G, Sudan I, Sudan II, Sudan III, Sudan Red 7B, Sudan IV) in animal tissues and eggs was developed. Samples were extracted with acetonitrile followed by a cleanup using a C(18) solid-phase extraction column. Chromatographic separation was achieved on a Zorbax SB-C(18) column under gradient conditions. The analytes were detected at 510 nm by HPLC with diode array detection. Specificity, decision limit (CCalpha), detection capacity (CCbeta), accuracy, and precision were determined during validation process. Recoveries for six Sudan dyes from three animal tissues and eggs were 77.2-98.0% with excellent relative standard deviations. CCalpha and CCbeta were in the range of 7.7-9.0 microg/kg and 9.1-10.3 microg/kg, respectively. The limits of quantitations were between 12.8 microg/kg and 15.0 microg/kg.
建立了一种简单、灵敏的高效液相色谱（HPLC）分析方法，用于同时测定动物组织和蛋类中的六种苏丹染料（苏丹红G、苏丹I、苏丹II、苏丹III、苏丹红7B、苏丹IV）。样品先用乙腈提取，然后使用C(18)固相萃取柱进行净化。在梯度条件下，于Zorbax SB-C(18)柱上实现色谱分离。通过配有二极管阵列检测的HPLC在510 nm波长处检测分析物。在验证过程中测定了特异性、决策限（CCalpha）、检测能力（CCbeta）、准确度和精密度。三种动物组织和蛋类中六种苏丹染料的回收率为77.2 - 98.0%，相对标准偏差优异。CCalpha和CCbeta分别在7.7 - 9.0微克/千克和9.1 - 10.3微克/千克范围内。定量限在12.8微克/千克至15.0微克/千克之间。
